Genome characterization of a new strain of peanut chlorotic streak virus causing chlorotic vein banding disease of groundnut (Arachis hypogaea L.) in India

Satyanarayana, T and Broglio, E P and Sreenivasulu, P and Reddy, D V R and Nayudu, M V (1995) Genome characterization of a new strain of peanut chlorotic streak virus causing chlorotic vein banding disease of groundnut (Arachis hypogaea L.) in India. Plant Pathology, 44 (6). pp. 966-970. ISSN 1365-3059

[img] PDF - Published Version
Restricted to ICRISAT users only

Download (316kB) | Request a copy

Abstract

The double-stranded DNA of the chlorotic vein banding isolate of peanut chlorotic streak caulimovirus (PC1SV-CVB), isolated from purified virus, resolved into circular and linear molecules similar to those of other caulimoviruses. A physical map of viral DNA was constructed, which showed the PCLSV-CVB DNA to be circular and composed of approximately 8.2 kbp. A number of restriction sites were found to be shared with a similar caulimovirus, PCLSV. Nevertheless, several differences between physical maps of the 2 viruses suggested that PCLSV-CVB should be considered as a distinct strain of PCLSV. Bam HI-cleaved PCLSV-CVB DNA was cloned into pUC 118 and was infectious when cleaved from the cloning vector and inoculated onto Vigna unguiculata [cowpeas].

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Subjects: Mandate crops > Groundnut
Depositing User: Library ICRISAT
Date Deposited: 27 Oct 2011 05:10
Last Modified: 27 Oct 2011 05:11
URI: http://oar.icrisat.org/id/eprint/2820
Official URL: http://dx.doi.org/10.1111/j.1365-3059.1995.tb02654...
Projects: UNSPECIFIED
Funders: Council of Scientific and Industrial Research(India)
Acknowledgement: The results presented in this paper were submitted as ICRISAT Journal Article No. 1601. T.S. thanks the Council of Scientific and Industrial Research, New Delhi, India, for providing a fellowship (in the form of CSIRSRF) during the period of research. The authors thank Dr R,J. Shepherd. University of Kentucky, Lexington, USA and Dr M.A. Mayo. Scottish Crop Research Institute, Invergowrie, Dundee, Scotland, UK, for critically reading the manuscript, and Dr R.D. Richins, University of California, Riverside, USA for generously providing the CaMV DNA
Links:
View Statistics

Actions (login required)

View Item View Item