Novel sources of resistance to blast disease in finger millet

Dida, M M and Oduori, C A and Manthi, S J and Avosa, M O and Mikwa, E O and Ojulong, H F and Odeny, D A (2021) Novel sources of resistance to blast disease in finger millet. Crop Science (TSI), 61 (1). pp. 250-262. ISSN 0011-183X

PDF - Published Version Download (1MB)

Abstract

Finger millet (Eleusine coracana(L.) Gaertn. subsp.coracana) is the most importantmillet in eastern Africa and perhaps the oldest domesticated cereal grain in Africa.One of the major factors limiting finger millet production is blast disease caused bythe fungusMagnaporthe grisea. Crop wild relatives and landraces present a poten-tial source of novel genes. This study investigated the response of cultivated and wildrelatives of finger millet to an isolate of blast disease from western Kenya. Previousgermplasm collections were purified through two generations of single-seed descentbefore screening alongside improved and farmer-preferred varieties (FPVs) under ascreen house across three seasons. Farmer-preferred varieties were identified throughparticipatory varietal selection (PVS). The plants were inoculated twice during eachgrowth period using hand-spraying method and data on disease incidence recorded atgrain-filling stage. Genotypic data was generated using diversity arrays technology(DArT) sequencing and data analysis done using Genstat 18.2 and TASSEL 5.2.58.We observed high heritability (81%), indicating that the variation observed was pre-dominantly genetic. Wild accessions were generally more resistant to the disease incomparison to the cultivated accessions. Preliminary genome-wide association study(GWAS) using general linear model with principal component analysis led to theidentification of 19 markers associated with blast disease that will be be developedinto assays for genotype quality control and trait introgression. Wild accessions andlandraces of finger millet present a good reservoir for novel genes that can be incor-porated into crop improvement programs.

Item Type:	Article
Divisions:	Research Program : East & Southern Africa
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Finger Millet, Cereals, Plant Disease
Subjects:	Others > Cereals Mandate crops > Millets > Finger Millet Others > Plant Disease
Depositing User:	Mr Arun S
Date Deposited:	29 Mar 2021 10:49
Last Modified:	29 Mar 2021 10:50
URI:	http://oar.icrisat.org/id/eprint/11769
Official URL:	https://doi.org/10.1002/csc2.20378
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	This work was undertaken as part of the initiative “Adapt-ing Agriculture to Climate Change: Collecting, Protecting andPreparing Crop Wild Relatives,” which is supported by theGovernment of Norway. The project is managed by the GlobalCrop Diversity Trust with the Millennium Seed Bank of theRoyal Botanic Gardens, Kew, and implemented in partnershipwith national and international gene banks and plant breeding institutes around the world.
Links:	Google Scholar

View Statistics

Actions (login required)

View Item