Characterization of potential probiotic bacteria isolated from sorghum and pearl millet of the semi-arid tropics

Kunchala, R and Banerjee, R and Mazumdar, S D and Durgalla, P and Srinivas, V and Gopalakrishnan, S (2016) Characterization of potential probiotic bacteria isolated from sorghum and pearl millet of the semi-arid tropics. African Journal of Biotechnology, 15 (16). pp. 613-621. ISSN 1684-5315

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The present study was conducted to isolate and characterize probiotic properties of bacteria isolated from flour and batter samples of sorghum and pearl millet. A total of five different selective media including plate count agar, yeast glucose chloramphenicol agar, Bifidobacterium agar, actinomycetes isolation agar and de ManRogosa and Sharpe agar were used and the most prominent bacteria (which were found abundantly in the plate) were isolated and maintained on the respective media slants at 4°C for further studies. The bacteria were characterized for various traits including Gram staining, morphology (color, size, shape, elevation, margin, form and surface), biochemistry (urease, catalase, oxidase, hydrogen sulphide, nitrogen reduction, gelatin liquefaction, starch hydrolysis and carbohydrate utilization), IMViC tests (indole, methyl red, Voges Proskauer and citrate utilization), probiotic potentials [acid (pH 2, 3), bile (0.5%), NaCl (6 and 9%)], phenol tolerance [0.4%], antibiotic tolerance (tetracycline, streptomycin, kanamycin, chloramphenicol, ciprofloxacin, ampicillin, penicillin, erythromycin and vancomycin) and antimicrobial activity against human pathogens (Escherichia coli, Staphylococcus aureus and Salmonella typhi). A total of nine probiotic bacterial isolates were short listed based on these traits. The sequences of 16s rDNA gene of the nine isolates were found matched with Bacillus subtilis (two isolates), Bacillus cereus (three isolates), Bacillus pumilus (one isolate), Bacillus amyloliquefaciens (one isolate), Sphingobacterium thalpophilum (one isolate) and Brevibacterium sp. (one isolate) in BLAST analysis. The sequences of the nine bacteria were submitted to NCBI and accession numbers obtained. This study indicated that the selected bacteria could be exploited to develop new probiotic foods.

Item Type: Article
Divisions: RP-Dryland Cereals
CRP: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: Probiotics, Prebiotics, Sorghum, Pearl millet, Product development
Subjects: Mandate crops > Millets > Pearl Millet
Mandate crops > Sorghum
Depositing User: Mr Ramesh K
Date Deposited: 18 Apr 2016 04:57
Last Modified: 21 Oct 2016 04:58
Official URL:
Acknowledgement: The authors are grateful to the Ministry of Science and Technology, Government of India for their financial assistance (INT/SL/12/P-001). This work was undertaken as part of the CGIAR Research Program on dry land cereals. ICRISAT is a member of CGIAR Consortium. The authors acknowledge Dr Jana Kholova and Dr SK Gupta from ICRISAT for kindly providing the sorghum and pearl millet samples used in this study, respectively. The authors would also like to thank ICRISAT and all of the staff members of the biocontrol unit, including PVS Prasad, P Manohar, B Nagappa, D Barath and A Jabbar for their technical assistance.
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