SSR Markers Linked with Alectra vogelii Resistance in Cowpea (Vigna unguiculata (L.) Walp)

Omoigui, L O and Ugba, M S and Bello, L L and Gowda, B S and Timlo, M P and Motagi, B N (2015) SSR Markers Linked with Alectra vogelii Resistance in Cowpea (Vigna unguiculata (L.) Walp). In: 5th International conference on Next Generation Genomics and Integrated Breeding for Crop Improvement (NGGIBCI-V), February 18 - 20, 2015, ICRISAT, Patancheru, Hyderabad.

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Cowpea [Vigna unguiculata (L.) Walp.] is an important food legume grown in tropical and subtropical regions of the world, primarily in Sub-Saharan Africa. Despite the importance of cowpea, yield on farmers' field is still low due to variety of biotic and abiotic stresses that constrain its production. Among the biotic constraints, the parasitic flowering plant, Alectra voge/ii (Benth.) is one of the more formidable limitations to cowpea production in the dry Savannas of West and Central Africa, a region which accounts for over 64 % of world cowpea production. Several control measures have been suggested for the control of the parasite. These include: cultural practices, application of ethylene chemicals, and host plant resistance. Among these control measures, the use of resistance cultivars appears to be the most attractive option to the resource poor farmers in sub-Saharan Africa Breeding resistance cultivars would be facilitated by markerassisted selection (MAS). The objective of this study was to identify molecular markers tightly linked to Alectra resistance gene that would be useful in MAS in breeding cowpea for resistance to Alectra vogelii. F2 population of a single cross, Banjar (susceptible parent) x B30 I (resistant parent) was screened for reaction to Alectra using pot culture technique. DNA was extracted from parental genotypes and F2 lines from young leaves of plant at 14 days after planting using FTA ® PlantSaver cards. 50 SSR cowpea, 40 SSR rice bean and 50 SSR asparagus bean primers, previously reported to give amplification products in cowpea, were used to screen DNA from B30 I and Banjar for polymorphism. Of the 140 primers screened 20 primers were polymorphic between B30 I and Banjar and these were used in the technique of BSA performed with DNA bulks of highly resistant and highly susceptible F2 lines to select those that cosegregate with the resistant gene. Two of the markers (RB 16 from rice bean and CLM0356 from asparagus bean) were found to be consistently associated with the resistance gene. The utility of these two markers were validated using 150 F21ines for marker segregation and association analysis. Similarity index (SI) revealed that these markers were closely linked (90.23%) with Alectra resistance gene. Cluster analysis as depicted by dendogram also showed a tight association (>0.75) between these markers, suggesting that these markers can be explored in MAS targeting breeding for Alectra resistance in cowpea.

Item Type: Conference or Workshop Item (Paper)
Divisions: RP-Grain Legumes
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: SSR Markers, Cowpea, Legumes, Africa
Subjects: Others > Food Legumes
Depositing User: Mr Ramesh K
Date Deposited: 05 Jan 2016 08:59
Last Modified: 05 Jan 2016 08:59
Acknowledgement: UNSPECIFIED
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