Development, characterization and utilization of microsatellite markers in pigeonpea

Odeny, D A and Jayashree, B and Ferguson, M and Hoisington, D A and Crouch, J H and Gebhard, C (2007) Development, characterization and utilization of microsatellite markers in pigeonpea. Plant Breeding, 126 (2). pp. 130-136.

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Pigeonpea is a major legume of the semi-arid tropics that has been neglected in terms of molecular breeding. The objectives of this study were to develop microsatellite markers and evaluate their potential for use in pigeonpea genetics and breeding. Two hundred and eight microsatellite loci were isolated by screening a non-enriched partial genomic library. Primers were designed for 39 microsatellite loci, 20 of which amplified polymerase chain reaction products of the expected size. Nineteen of the primer pairs were polymorphic amongst 15 cultivated and nine wild pigeonpea accessions providing evidence for cross-species transferability within the genus Cajanus. A total of 98 alleles were detected at the 19 polymorphic loci with an average of 4.9 alleles per locus. The observed heterozygosity ranged from 0.17 to 0.80 with a mean of 0.42 per locus. Less allelic variation (31 alleles) was observed within the cultivated species than across the wild species (92 alleles). The diversity analysis readily distinguished all wild relatives from each other and from the cultivated germplasm. Development of more microsatellites is recommended for future genomic studies in pigeonpea.

Item Type: Article
Subjects: Mandate crops > Pigeonpea
Depositing User: Library ICRISAT
Date Deposited: 04 Sep 2011 18:10
Last Modified: 10 Apr 2012 10:08
Official URL:
Acknowledgement: The authors thankDr Agim Ballvora andMs BirgitWalkemeier (MPIZ) for their assistance in genomic library preparation. Many thanks to ICRISAT’sMs SeethaKannan for providingDNAof thewild species,Dr H. Upadhyaya for selecting and providing seeds of diverse genotypes and Mr Prasanth V.P. for statistical analysis. The authors gratefully acknowledge the leading role of Prof. Dorothea Bartels (University of Bonn) in co-ordinating this work and Prof. Paul Vlek (Centre for Development Research, Bonn, Germany) for organizing funds for this project.
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