Horizontal Gene Transfer Through Genetic Transformation

Bhatnagar-Mathur, P and Palit, P and Sharma, K K (2014) Horizontal Gene Transfer Through Genetic Transformation. In: Alien Gene Transfer in Crop Plants: Innovations, Methods and Risk Assessment. Springer, New York, pp. 105-141. ISBN 978-1-4614-9572-7

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Gene transfer technology in crop plants has tremendous potential to introduce newer and better traits through development of transgenics and broaden the genetic base of crop plants by transferring genes from novel sources overcoming the species and genus barriers. Nevertheless, development of efficient transformation systems remains a prerequisite and might involve many years of exhaustive research. This chapter overviews the different methods of alien gene transfer through genetic transformation and factors affecting efficient transformation across different crop species. A comparative study on Agrobacterium and biolistics-mediated transformation including methods for production of marker-free transgenics are described in detail. Addressing the growing concerns over the biosafety issue constraining wider application of GM products in agriculture this chapter also focuses on improved methods of choice with respect to a crop family and also deals with future strategies which can help in further exploiting the existing technologies to develop improved crop varieties which can help to combat poverty, hunger and global agro-climatic changes.

Item Type: Book Section
Divisions: RP-Grain Legumes
Uncontrolled Keywords: Agrobacterium-mediated transformation, Biolisitcs, Co-transformation, Gene transfer, Marker-free transgenics, Selectable marker, Transgene escape,
Subjects: Others > Genetics and Genomics
Depositing User: Mr Siva Shankar
Date Deposited: 12 Feb 2014 04:49
Last Modified: 12 Feb 2014 04:49
URI: http://oar.icrisat.org/id/eprint/7505
Acknowledgement: UNSPECIFIED
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