Mapping and identification of a Cicer arietinum NSP2 gene involved in nodulation pathway

Ali, L and Madrid, E and Varshney, R K and Azam, S and Millan, T and Rubio, J and Gill, J (2014) Mapping and identification of a Cicer arietinum NSP2 gene involved in nodulation pathway. Theoretical and Applied Genetics, 127 (2). pp. 481-488. ISSN 0040-5752

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An intraspecific cross between the mutant non-nodulating genotype PM233, carrying the recessive gene rn1, and the wild-type CA2139 was used to develop two pairs of near-isogenic lines (NILs) for nodulation in chickpea. These pairs of NILs were characterized using sequence tagged microsatellite site (STMS) markers distributed across different linkage groups (LGs) of the chickpea genetic map leading to the detection of polymorphic markers located in LG5. Using this information, together with the genome annotation in Medicago truncatula, a candidate gene (NSP2) known to be involved in nodulation pathway was selected for mapping in chickpea. The full length sequence obtained in chickpea wild-type (CaNSP2) was 1,503 bp. Linkage analysis in an F3 population of 118 plants derived from the cross between the pair of NILS NIL7-2A (nod) × NIL7-2B (non-nod) revealed a co-localization between CaNSP2 and Rn1 gene. These data implicate the CaNSP2 gene as a candidate for identity to Rn1, and suggest that it could act in the nodulation signaling transduction pathway similarly to that in other legumes species.

Item Type: Article
Divisions: RP-Grain Legumes
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: legumes species, Cicer arietinum, Chickpea
Subjects: Mandate crops > Chickpea
Others > Genetics and Genomics
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 21 Nov 2013 04:59
Last Modified: 19 Aug 2016 04:40
Official URL:
Acknowledgement: UNSPECIFIED
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