Comparison of molecular markers for developing pearl millet genetic linkage maps

Murali Krishna, I (1997) Comparison of molecular markers for developing pearl millet genetic linkage maps. Masters thesis, Anna University.

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Supervisors NameSupervisors ID
Seetharama, NICRISAT
Kaliraj, PAnna University


Downy mildew (DM) disease caused by the fungus Sclerosprora graminicola (Sacc.) Schroet is the major yield reducer of pearl millet cropped in India and Africa. DM resistance is a complex, quantitatively - inherited trait. The available RFLP map of this crop developed at the Cambridge laboratory at Norwich, UK is useful for breeding for DM resistant pearl m~llets by markerassisted selection and backcrossing. Further, the quantitative trait loci (QTLs) identified for resistance are also useful for searching for newer sources of resistance. The present work deals with identification of newer sources and markers for DM resistance in pearl millet. For this various types of molecular markers were compared. The marker types being compared includs Isozymes, RFLP, RAPD, and AFLP. Among the five accessions of pearl millet selected for the study three (7042 DMR P2 PI, iP 18298, and lP18292) are downy m~ldew res~stant and the other two accessions (8438 P5 P2, and 23 D2B ) are downy mildew suseptible. Out of the eight isozyme systems tried three (peroxidase, esterase, alcohol dehydrogenase) detected polymorphism between the test entries. RFLP analysis of these five accessions with the homologous genomic clones (PSM) in combination with 2 to 4 restriction enzymes (Dral, Hindlll, EcoRI, EcoRV) have revealed several polymorphic bands. RAPD assays with the same pearl millet accessions and 16 random primers showed that 50% of the RAPD primers identified one or more polymorphic bands. AFLP assay of two accessions 843 B PS P1 and 7042 DMR P2 PI revealed several bands in a 5% denaturing polyactylamide gel. All the seven primers tested showed polymorphism. AFLP seem to be technique of choice for further characterization of power of resistance and genetic linkage mapping.

Item Type: Thesis (Masters)
Subjects: Mandate crops > Millets
Depositing User: Users 6 not found.
Date Deposited: 30 Aug 2011 05:28
Last Modified: 30 Aug 2011 05:29
Acknowledgement: I take this opportunity to express my deep sense of gratitude to my supervisor, Dr. NSeetharama, senior scientist at ICRISAT for h~s valuable guidance during my investigation and in writing the thesis. I express my sincere gratitude to Prof. Kunthala Jayaraman, Dean, Faculty of Technology, Anna University and Prof. P. Kaliraj, Director, Centre For Biotechnology for allowing me to work on this project. I am also grateful to Dr. Manjula Sritharan and Dr. R.B.Narayanan for their help in getting me this project. I like to express my gratefulness to Dr. Diwakar, Program Leader, TAFP and to Mr. K.D.V Prasad, Design and Development Division, ICRISAT for the~r help in carrying out my work at ICRISAT. I wish to express my heartfelt gratitude and indebtedness to Leela, Research Associate, and Mr. Yosuf, CMBD, ICRISAT without whose help th~s work might not be in its present form. I wish to specially thank Shyamala, Dr. Sairam, Dr. Hiren, Srinivas, Anand and Sravan for their help and valuable suggestions. I specially thank Hanumantha Rao, Prashanth, Laxminarayana, Malla Reddy, and others in CMBD lab. I also thank my friends Erik, Raj, Leela Prasad and other classmates for their help.
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