Genetic diversity analysis, QTL mapping and marker-assisted selection for shoot fly resistance in sorghum [Sorghum bicolor (L,) Moench]

Mehtre, S P (2006) Genetic diversity analysis, QTL mapping and marker-assisted selection for shoot fly resistance in sorghum [Sorghum bicolor (L,) Moench]. PHD thesis, Marathwada Agricultural University, Parbhani.

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Supervisors NameSupervisors ID
Borikar, S TMarathwada Agricultural University


The present investigation entitled. "Genetic diversity analysis, QTL mapping and marker-assisted selectiocl for shoot fly resistance in sorgllum [Sorghl~rrr hicolor (L.) Moench]" was aimed, 1) to assess genetic dhersity by SSR markers in a set of insect resistant lines. 2) to understand tlie genetics of tlie shoot fly resistance and locnte the chromosomal regions harboring the quantitative trait loci (QTLs) for shoot fly resistance and related component traits. and 3) introgression of shoot fly resistance co~nponents traits in agronomically superior genotypes using ~nolecular markerassisted selection. For all the sti~dics involving the use ol' SSIi ~narkers, DNA was extracted with a modified CLAD metllod. 111 the first ubjective. genetic diversity analysis by SSK markers in a sct of sorghulu lines. 91 sorghu~n accessions were genotyped using 20 SSIi primel- pairs that detected loci distributed over 9 of the 10 linkage groi~ps ill the sorgliuni ~iuclenr genome. PCR amplilication of a targeted sequence was conducted in an Applied Biosysteln GecleALIP I'CK systeni 0700 tliermocycler using a touch do~vn PCK pl.otocril. Ampliiied products were separated by electrophoresis on 6% non-denaturing polyncr)lamide gels. and by capillar). electsophoresis using automated DNA scqucnccrs. PCR products separated by PAGE \\ere visualized by silver staining. Illtensely amplified specilic bands representing the corresponding allclic products oS thc SSR 111arliel.s in each accession \bere scored to produce tlie PAGE data set. 'l'hc N'I'SYS statistical solinare package was used for cluster atialysis of the SSR niarker allele data sets f~omth e PAGE and capillary electrophoresis I'CR product sepalation ~netliods. Jaccard's siciiilarity coefficient bet\veen each pair of accessions was used to construct de~idrogra~nfosr each of these two data sets usi~ig the un-weighted paired group method with arithmetic averages (UPGMA)...

Item Type: Thesis (PHD)
Subjects: Mandate crops > Sorghum
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 21 Feb 2013 04:40
Last Modified: 16 Sep 2013 09:45
Acknowledgement: UNSPECIFIED
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