Next-generation sequencing technologies and their implications for crop genetics and breeding

Varshney, R K and Nayak, S N and May, G D and Jackson, S A (2009) Next-generation sequencing technologies and their implications for crop genetics and breeding. Trends in Biotechnology, 27 (9). pp. 522-530.

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Using next-generation sequencing technologies it is possible to resequence entire plant genomes or sample entire transcriptomes more efficiently and economically and in greater depth than ever before. Rather than sequencing individual genomes, we envision the sequencing of hundreds or even thousands of related genomes to sample genetic diversity within and between germplasm pools. Identification and tracking of genetic variation are now so efficient and precise that thousands of variants can be tracked within large populations. In this review, we outline some important areas such as the large-scale development of molecular markers for linkage mapping, association mapping, wide crosses and alien introgression, epigenetic modifications, transcript profiling, population genetics and de novo genome/organellar genome assembly for which these technologies are expected to advance crop genetics and breeding, leading to crop improvement.

Item Type: Article
Subjects: Others > Agriculture-Farming, Production, Technology, Economics
Depositing User: Library ICRISAT
Date Deposited: 26 Aug 2011 12:09
Last Modified: 01 Sep 2011 12:56
Official URL:
Funders: Generation Challenge Programme, Indian Council of Agricultural Research, National Science Foundation (NSF), USA, Government of India - Department of Biotechnology
Acknowledgement: We thank the Generation Challenge Programme (GCP) (R.K.V., G.D.M.), the National Science Foundation (DBI 0501877 and 0227414 to S.A.J.), the Pigeonpea Genomics Initiative of the Indian Council of Agricultural Research (ICAR) under the umbrella of the Indo–US Agricultural Knowledge Initiative (AKI) (R.K.V.), the Department of Biotechnology (R.K.V.) and the Council of Scientific and Industrial Research (for a fellowship to S.N.N.) of the Government of India for funding research in our laboratories. Thanks are also due to B. Jayashree, V. Thakur and the anonymous reviewers for their useful suggestions on earlier versions of the manuscript.
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