The first SSR-based genetic linkage map for cultivated groundnut (Arachis hypogaea L.)

Varshney, R K and Bertioli, D J and Moretzsohn, M C and Vadez, V and Krishnamurthy, L and Aruna, R and Nigam, S N and Moss, B J and Kannan, S and Ravi, K and He, G and Knapp, S J and Hoisington, D A (2009) The first SSR-based genetic linkage map for cultivated groundnut (Arachis hypogaea L.). TAG Theoretical and Applied Genetics, 118 (4). pp. 729-739.

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Molecular markers and genetic linkage maps are pre-requisites for molecular breeding in any crop species. In case of peanut or groundnut (Arachis hypogaea L.), an amphidiploid (4X) species, not a single genetic map is, however, available based on a mapping population derived from cultivated genotypes. In order to develop a genetic linkage map for tetraploid cultivated groundnut, a total of 1,145 microsatellite or simple sequence repeat (SSR) markers available in public domain as well as unpublished markers from several sources were screened on two genotypes, TAG 24 and ICGV 86031 that are parents of a recombinant inbred line mapping population. As a result, 144 (12.6%) polymorphic markers were identified and these amplified a total of 150 loci. A total of 135 SSR loci could be mapped into 22 linkage groups (LGs). While six LGs had only two SSR loci, the other LGs contained 3 (LG_AhXV) to 15 (LG_AhVIII) loci. As the mapping population used for developing the genetic map segregates for drought tolerance traits, phenotyping data obtained for transpiration, transpiration efficiency, specific leaf area and SPAD chlorophyll meter reading (SCMR) for 2 years were analyzed together with genotyping data. Although, 2-5 QTLs for each trait mentioned above were identified, the phenotypic variation explained by these QTLs was in the range of 3.5-14.1%. In addition, alignment of two linkage groups (LGs) (LG_AhIII and LG_AhVI) of the developed genetic map was shown with available genetic maps of AA diploid genome of groundnut and Lotus and Medicago. The present study reports the construction of the first genetic map for cultivated groundnut and demonstrates its utility for molecular mapping of QTLs controlling drought tolerance related traits as well as establishing relationships with diploid AA genome of groundnut and model legume genome species. Therefore, the map should be useful for the community for a variety of applications.

Item Type: Article
Subjects: Others > Agriculture-Farming, Production, Technology, Economics
Depositing User: Library ICRISAT
Date Deposited: 26 Aug 2011 12:07
Last Modified: 13 Sep 2011 06:21
Official URL:
Funders: Indian Council of Agricultural Research, Generation Challenge Programme
Acknowledgement: Authors are thankful to Mr A Gafoor and Mr G Somaraju for their help in conducting some experiments and collection of data. Financial support from National Fund of Indian Council of Agricultural Research (NBFSRA), New Delhi, India and Generation Challenge Programme ( of CGIAR is gratefully acknowledged to sponsor this study.
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