Double Haploids in Pearl Millet: Major Project Report Submitted in Partial Fulfillment of the Requirement for the Degree Bachelor of Technology

Niharika, T (2012) Double Haploids in Pearl Millet: Major Project Report Submitted in Partial Fulfillment of the Requirement for the Degree Bachelor of Technology. Other thesis, Joginpally B.R Engineering College.

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Supervisors NameSupervisors ID
Mallikarjuna, NaliniICRISAT


Anther culture is an important technique to generate double haploid plants. Doubled haploids would help to produce homozygous lines in single step which accelerates the breeding programmes. They are also useful for studying mutagenesis, dosage effects and interaction & linkage of genes. Pearl millet is a diploid (2n=14) plant and summer crop that originated from west Africa. It has become a challenge to develop a reproducible protocol for double haploids in pearl millet. For culture initiation it is necessary to select florets and anthers with appropriate developmental stage of microspore development. The optimal stage for pearl millet anther culture development has been found to be when the microspores are uninucleate with the nucleus in the center and when the spike is still enclosed in the flag leaf sheath. The growth of the donor plants and developmental stage of microspores have been important factors in the intiation of anther cultures. The present study aims to assess the effect of different factors such as growth hormones, media, pretreatments on the induction of androgenesis (division of microspores) and to develop a suitable protocol for producing embryoids of pearl millet in vitro using floret and anther culture.

Item Type: Thesis (Other)
Subjects: Mandate crops > Millets
Others > Genetics and Genomics
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 24 May 2012 09:18
Last Modified: 20 Nov 2015 05:03
Acknowledgement: My sincere thanks to ICRISAT for providing an opportunity to work in this esteemed organization. I would like to thank Dr.Nalini Mallikarjuna, for her guidance and support in wide crosses and cell biology laboratory, ICRISAT. I am eternally grateful to her. I am thankful to Dr. DEEPAK JADHAV,Scientific officer, wide crosses and cell biology laboratory for his support. I spcialy thank all students and members of the lab for providing constant support and the tips they shared from thir experiences. I thank Prof G Venkateshwarulu, head of dept. of Biotechnology, Dr. N Suneetha, HOD-in-charge and Dr.Zehra Siddiqui, project coordinator for allowing me to do the project outside the college and for their support and encouragement. I would like to thank my internal guide G.Srinivas, assistant professor for his support and valuable suggestions for the completion of my project. I would like to thank the other faculty at college for their support and suggestions. I am sure that this is not the complete list. I thank one and all for their help.
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