Efficiency of three DNA markers in revealing genetic variation among wild Cajanus species

Aruna, R and Rao, D M and Sivaramakrishnan, S and Reddy, L J and Bramel, P and Upadhyaya, H D (2009) Efficiency of three DNA markers in revealing genetic variation among wild Cajanus species. Plant Genetic Resources, 7 (2). pp. 113-121.

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Wild relatives of pigeonpea (Cajanus cajan L.) possess many useful genes that can be utilized for crop improvement, most importantly genes for resistance to Helicoverpa armigera, the legume pod borer. The present study aimed at quantifying diversity in a collection of Cajanus scarabaeoides, Cajanus sericeus, Cajanus reticulatus and C. cajan species selected from a wide geographic range using two PCR-based marker systems, amplified fragment length polymorphism (AFLP) and simple sequence repeats (SSRs), and the hybridization-based restriction fragment length polymorphism (RFLP). Polymorphism was higher among the wild accessions than among the cultivated genotypes. Wild and cultivated Cajanus accessions belonging to different species clustered into four distinct major groups largely based on the interspecific differences. C. scarabaeoides accessions derived from same geographical origins formed one group reflecting similar genetic makeup of these accessions. Dendrograms generated using AFLP, RFLP and SSR marker data were comparable with minor clustering differences, which suggests that either method, or a combination of both can be applied to expanded genetic studies in Cajanus. Mantel testing confirmed the congruence between the genetic distances of three markers, indicating that the markers segregated independently, giving similar grouping patterns of all accessions having similar genetic origin.

Item Type: Article
Subjects: Mandate crops > Pigeonpea
Depositing User: Library ICRISAT
Date Deposited: 26 Aug 2011 10:58
Last Modified: 15 Mar 2012 04:05
URI: http://oar.icrisat.org/id/eprint/591
Official URL: http://dx.doi.org/10.1017/S1479262108061479
Funders: Department for International Development
Acknowledgement: The first author expresses her gratitude to the APNL – Biotechnology unit for providing fellowship, Department for international Development for funds and ICRISAT for the infrastructure facilities. We gratefully acknowledge the valuable comments on the manuscript by Dr S. N. Nigam. We acknowledge the able valuable guidance by Ms K Seetha in laboratory, Mr Hari in statistical analysis and technical assistance of Mr Narsi Reddy and Mr R. Luke.
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