A survey of restriction fragment length polymorphisms in tall fescue and its relatives

Xu, W W and Sleper, D A and Hoisington, D A (1991) A survey of restriction fragment length polymorphisms in tall fescue and its relatives. Genome, 34 (5). pp. 686-692. ISSN 1480-3321

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Restriction fragment length polymorphisms (RFLPs) have several advantages over conventional genetic markers and as a result have received increased attention from plant breeders and geneticists. The objective of this study was to construct a tall fescue (Festuca arundinacea Schreb.) genomic library and to survey RFLPs in tall fescue and its relatives. Using plasmid pUC19 as a vector and Escherichia coli XL1-Blue cells as hosts, the first reported PstI genomic DNA library has been established from hexaploid (2n = 6x = 42) tall fescue. The genomic clones were evaluated using nine genotypes from three species of Festuca and three restriction enzymes (BamHI, EcoRI, and HindIII). One hundred and seventy-four probes gave readable results, of which 21% were repetitive and 79% single-copy. The single-copy probes revealed good polymorphism in tall fescue. Approximately 21% of the probes did not cross hybridize to any of the diploids or tetraploids or both and, therefore, represented genome-specific clones

Item Type: Article
Uncontrolled Keywords: tall fescue, genome-specific probes, genomic library, polyploids, genomic relationships
Subjects: Others > Genetics and Genomics
Depositing User: Mr Siva Shankar
Date Deposited: 20 Mar 2012 04:39
Last Modified: 20 Mar 2012 04:39
URI: http://oar.icrisat.org/id/eprint/5766
Official URL: http://dx.doi.org/10.1139/g91-106
Acknowledgement: [NOTE: D. A. Hoisington is presently with ICRISAT (Patancheru)]---The authors thank Mr. J. Gardiner for technical assistance and Dr. G. Eiezenga for providing the diploid cultivars.
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