The Occurrence Of Yellow Spot Virus, A Member Of Tomato Spotted Wilt Virus Group, On Peanut (Arachis Hypogaea L) In India

Reddy, D V R and Sudharshana, M R and Ratna, A S and Reddy, A S and Amin, P W and Kumar, I K and Murthy, A K (1991) The Occurrence Of Yellow Spot Virus, A Member Of Tomato Spotted Wilt Virus Group, On Peanut (Arachis Hypogaea L) In India. In: USDA Workshop "Virus-Thrips-Plant Interactions of Tomato Spotted Wilt Virus,", April 18-19 1990, Beltsville, Maryland.

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A virue that induced yellow leaf spots which later coalesced and became necrotic, vae ieolated from peanut (bacua -1 in India and named peanut yellow apot'virus (PYSY). PYSV was sap-transmisslbls to eleven epecles of Chenopodiaccao. Lcpualnoeac, and Salanacsaa. The virus induced local leslona in all tho host., Y. uaa a good local lesion host. The infection In Fl&m -. Ylnnn rndLata and Y. became ay~temic hen they uers maintained st temperatures between 20 and 30'~. In thin sectlone of peanut leaves rpherloal membrane-bound particlea of TO to 100 nm in diameter were observed to occur in cluatera. Purlfled virus Preparations contained five polypeptldeo of 90000. 54000. 44000. 40000 and 31000 dslton polypeptldca. Whan virus particle^ were treated vlth Nanldet P 40, the polypeptides ware removed with the exception of that of 31000 daltana In both ELISA end western blot., PYSV did not react vlth antisera to two tomato apotted wilt virus isolates. On the basis of these properties. PYSV is regarded as a previously undeecribed virus belon(linr to the tomato spotted wilt vlrus group.

Item Type: Conference or Workshop Item (Paper)
Subjects: Mandate crops > Groundnut
Depositing User: Library ICRISAT
Date Deposited: 04 Dec 2011 09:03
Last Modified: 04 Dec 2011 09:03
Acknowledgement: We are grateful to Dr. D, HcDonald, Prograa Director, Legumes, for crltlcally reading this manuscript. We viah to thank Dra. A.M. Chanekbr, N. Bharathan and Mrs. R. Rejeehvarl for rendering help in the initial stage0 of thle vork. We thank Hr 5. Shyameundar Rao for help with machsnlcal sap inoculations and Wrs 1 Radha in the preparation of thin manuscript.
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