Embryo Rescue in Wide Crosses in Arachis. 1. Culture of Ovules in Peg Tips of Arachis hypogaea

Moss, J P and Stalker, H T and Pattee, H E (1988) Embryo Rescue in Wide Crosses in Arachis. 1. Culture of Ovules in Peg Tips of Arachis hypogaea. Annals of Botany, 61 (1). pp. 1-7. ISSN 0305-7364

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Interspecific hybridization in Arachis is restricted by early embryo abortion for many cross-combinations. Rescue of young embryos in vitro within a week after fertilization is necessary to recover these embryos before they abort. Peg tips, with the ovule and embryo tissues, of A. hypogaea L. cv. ‘NC 6’, were cultured to compare ovule growth, callus production and peg elongation. Tissues were collected 1, 2, 3 and 4 d after self-pollination, after which peg meristems were removed from half the pegs and cultured on five media combinations. One-day-old pegs had significantly (P = 0.01) more ovule growth than older tissues. Presence of the meristem had a greater inhibition to ovule growth for 2- to 4-d pegs than for 1-d-old pegs. Significantly more callus was produced on 4-d pegs than younger tissues, and kinetin had the greatest stimulatory effect on callus. Elongation of pegs with the meristem attached was observed most often in media with high sucrose levels. The observations indicate that very young ovules can be grown in vitro, and techniques may be applicable to rescue of young embryonic tissues of Arachis

Item Type: Article
Uncontrolled Keywords: Ovule culture, interspecific hybridization, Arachis hypogaea, peanuts
Subjects: Mandate crops > Groundnut
Depositing User: Ms K Syamalamba
Date Deposited: 29 Nov 2011 10:11
Last Modified: 29 Nov 2011 10:11
URI: http://oar.icrisat.org/id/eprint/4466
Official URL: http://aob.oxfordjournals.org/content/61/1/1.abstr...
Funders: USAID Peanut CRSP
Acknowledgement: Submitted as paper no.603 of the journal series of the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) and paper no.10403 of the journal series of the North Carolina Agricultural Research Service, Raleigh, NC 27695-7601. Research was conducted by the senior author while on sabbatical leave at N.C. State University. Use of trade names of specific materials does not constitute endorsement by the United States Department of Agriculture or the North Carolina Agricultural Research Service to the exclusion of others which also may be available. This investigation was supported in part by USAID Peanut CRSP grant no.DAN-4048-G-SS-2065-00
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