Rapid Methods for Estimating Protein and Lysine in Sorghum (Sorghum bicolor (L.) Moench).

Jambunathan, R and Rao, N S and Gurtu, S (1982) Rapid Methods for Estimating Protein and Lysine in Sorghum (Sorghum bicolor (L.) Moench). Cereal Chemistry, 60. pp. 192-194. ISSN 0009-0352

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Sorghum (Sorghum bicolor (L.) Moench) samples were analyzed for protein by the Technicon auto analyzer (TAA) method, and the results were compared with the micro-Kjeldahl method. The TAA method was highly and significantly correlated (r = 0.997**) with the micro-Kjeldahl method, and the results were found to be highly reliable for routine screening of samples. Lysine was estimated by the rapid dye-binding capacity (DBC) procedure and was determined using an amino-acid analyzer. The results of the DBC procedure, when expressed as a ratio with respect to the protein content, were highly and positively correlated (r = 0.933**) with the amino-acid analyzer values. The rapid procedures of TAA and DBC were observed to give reliable estimates of protein and lysine content, respectively, and are recommended when large numbers of sorghum samples are to be analyzed.

Item Type: Article
Subjects: Mandate crops > Sorghum
Depositing User: Mr Charan Sai Ch
Date Deposited: 13 Nov 2011 11:56
Last Modified: 13 Nov 2011 11:56
URI: http://oar.icrisat.org/id/eprint/3369
Official URL: http://www.aaccnet.org/cerealchemistry/abstracts/1...
Acknowledgement: UNSPECIFIED
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