Development and Utilization of Genetic Diversity Based Ethiopian Chickpea (Cicer arietinum L.) Germplasm Core Collection for Association Mapping

Kibret, K T (2011) Development and Utilization of Genetic Diversity Based Ethiopian Chickpea (Cicer arietinum L.) Germplasm Core Collection for Association Mapping. PHD thesis, International Crops Research Institute for the Semi-Arid Tropics.

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Chickpea (Cicer arietinum L) is one of the most important cool season grain legume crops grown in semi- arid tropics and Mediterranean regions. Terminal drought stress is one of the limiting factors for chickpea production. Utilizing of germplasm collections are the main gateway to improve the stagnant production of chickpea in semi arid tropics. Hence, the objectives of this study were to i) Preliminary phenotyping and genotyping of germplasms collections for diversity assessment; ii) Development of chickpea core collection based on diversity analysis; iii) Identification of desirable accessions for drought tolerance from core set by proper phenotyping; iv) Large scale genotyping of the core collections by SNP markers; v) Large scale genotyping of the core collections by SNP markers; vi) Identification and establishing marker trait associations using appropriate association genetic approaches; vii) Quantification of population structure and relationship of Ethiopian chickpea collection. The phenotypic evaluation in contrasting environment and SNP marker data analysis revealed that there is significant phenotypic and genotypic variability in Ethiopian chickpea germplasm for drought tolerance and other agronomic traits. The population structure and relationship analysis also revealed strong subpopulation fixation and differentiation which was significantly different from the original population. High allelic and gene diversity were observed in the entire collection with common and rare alleles. Trait marker association analysis showed markers which are strongly associated with maturity related traits and high linkage disequilibrium observed for the polymorphic markers. Core collection for Ethiopian chickpea germplasm were developed and validated for different validation parameters such as percent mean difference (MD %), percent variance difference (VD %), analysis of variance, coincidence rate of range (CR %), variable rate of coefficient of variance (VR %) and genetic diversity index. The result of validation showed better correspondence between the core set and the entire set which had avoided germplasm duplication and representing the whole collection economically in time and money with few numbers of accessions. Drought tolerant accessions were also identified in the preliminary field screening which needs further confirmation.

Item Type: Thesis (PHD)
Subjects: Mandate crops > Chickpea
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 19 Oct 2011 12:03
Last Modified: 20 Nov 2015 05:11
Acknowledgement: It is sometimes difficult to express gratitude in few words because the feeling go beyond these. These three years of my Ph.D. work in Ethiopia and ICRISAT- India have changed the course of my thoughts and I have rediscovered myself with the help of some brilliant people I have been associated with. Firstly, I would like to thank my brilliant supervisor, director Dr. Rajeev K. Varshney for his excellent and unlimited guidance, encouragement, motivation, monitoring and financial support throughout the course of this study. I wish to thank my other supervisors, Principal scientist Dr Pooran Gaur, Asso. Prof. Firew Mekibib, and Principal scientist Dr Seid Ahmed Kemal for their guidance, encouragement and support throughout the study period. I would like to extend my thanks to senior scientist Dr Abhishek Rathory, Dr Mahender Thudi and Mr Anil Kumar helping in data analysis and continues encouragement. I am very grateful to Dr Rozan Mulla and other learning system unit members helping me in facilitating my arrival, encouraging every time in ICRISAT and my safe departure to my country. I appreciate the friendship, guidance of all my lab colleagues, in particular to Mr Abdul Gafoor, Mr Aziz and Mr B.J. Mose for their help in laboratory and green house work. I am also very grateful to ICRISAT, Sirinka Agriculture Research Center, Wollo University and Haramaya University supporting this finding financially and allowing me to work and stay throughout the course of the study in Ethiopia. I thank Institute of Biodiversity and Conservation (IBC), Ethiopia for giving enough germplasm for this research work especially Dr Tasfaye Wasso who helped me in getting planting material within short period of time. I am indebted to my family, in particular my wife Zenebech Mekonen and my sister Sehin Desalegn supporting me in every ups and downs, encouraging me in every time to finish this work on time.
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