A Pictorial Guide for the Identification of Mold Fungi on Sorghum Grain

Navi, S S and Bandyopadhyay, R and Hall, A J and Bramel-Cox, P J (1999) A Pictorial Guide for the Identification of Mold Fungi on Sorghum Grain. Information Bulletin (59). International Crops Research Institute for Semi Arid Tropics , Patancheru, Andhra Pradesh, India. ISBN 92-9066-416-9

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Sorghum is one of the main staple food crops of the world's poorest and most food-insecure people. Approximately 90% of the world's sorghum areas are located in Africa and Asia. During 1992-94, 42% of the total sorghum produced worldwide was utilized for food, and 48% for animal feed. A preliminary study was conducted to understand the various storage conditions of sorghum grain, and the potential occurrence of mold fungi under such conditions. A total of 67 sorghum grain samples were collected from two surveys, 15 samples from the 1996 rainy season harvest, and 11 from the 1996/97 postrainy season harvest collected in June 1997, and 19 samples from the 1996/97 postrainy season and 22 from 1997 rainy season harvest collected in October 1997. Approximately 1 kg grain from each of the grain lots stored under various conditions (gunny bags, mud-lined baskets, metallic containers, polypropylene bags, and grains piled in a corner of a room) by farmers in rural India was collected. Each grain sample (200 grains treatment1) was examined to identify fungi up to the species level. Grains with and without surface sterilization were transferred separately to pre-sterilized petri dish humid chambers under aseptic conditions. The petri dishes were incubated for 5 days at 28±1 °C in an incubator with a 12-h light cycle. Under each treatment, 200 grains (25 grains dish-1) were examined for 49 mold fungi, including the species of Aspergillus and Penicillium. The major fungi observed on the grains included species of Alternaria, Curvularia, Drechslera, Fusarium, and Rhizopus. The frequency of occurrence of the various fungi on each grain sample under the various treatments was analyzed. This bulletin reports some new mold fungi on sorghum grain in India: Alternaria longipes, Bipolaris zeicola, Curvularia affinis, C. clavata, C. fallax, C. geniculata, C. harveyi, C. ovoidea, C. pallescens, C. tuberculata, Drechslera halodes, Gonatobotrys simplex, Nigrospora oryzae, Periconia macrospinosa, Spadicoides obovata, Torula graminis, and Trichothecium roseum.

Item Type: Book
Series Name: Information Bulletin
Subjects: Mandate crops > Sorghum
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 27 Sep 2011 08:29
Last Modified: 07 Feb 2014 06:27
URI: http://oar.icrisat.org/id/eprint/1948
Acknowledgement: This publication is an output from two research projects funded by the United Kingdom Department for International Development (DFID) for the benefit of developing countries. The views expressed are not necessarily those of DFID [R6767, R7506, the Crop Protection Programme, and the Crop Post-Harvest Programme].
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