Gene expression and DNA methylation altering lead to the high oil content in wild allotetraploid peanut (A. monticola)

Liu, N and Wu, B and Pandey, M K and Huang, L and Luo, H and Chen, Y and Zhou, X and Chen, W and Huai, D and Yu, B and Chen, H and Guo, J and Lei, Y and Liao, B and Varshney, R K and Jiang, H (2022) Gene expression and DNA methylation altering lead to the high oil content in wild allotetraploid peanut (A. monticola). Frontiers in Plant Science (TSI), 13. 01-17. ISSN 1664-462X

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Introduction: The wild allotetraploid peanut Arachis monticola contains a higher oil content than the cultivated allotetraploid Arachis hypogaea. Besides the fact that increasing oil content is the most important peanut breeding objective, a proper understanding of its molecular mechanism controlling oil accumulation is still lacking. Methods: We investigated this aspect by performing comparative transcriptomics from developing seeds between three wild and five cultivated peanut varieties. Results: The analyses not only showed species-specific grouping transcriptional profiles but also detected two gene clusters with divergent expression patterns between two species enriched in lipid metabolism. Further analysis revealed that expression alteration of lipid metabolic genes with coexpressed transcription factors in wild peanut led to enhanced activity of oil biogenesis and retarded the rate of lipid degradation. In addition, bisulfite sequencing was conducted to characterize the variation of DNA methylation between wild allotetraploid (245, WH 10025) and cultivated allotetraploid (Z16, Zhh 7720) genotypes. CG and CHG context methylation was found to antagonistically correlate with gene expression during seed development. Differentially methylated region analysis and transgenic assay further illustrated that variations of DNA methylation between wild and cultivated peanuts could affect the oil content via altering the expression of peroxisomal acyl transporter protein (Araip.H6S1B). Discussion: From the results, we deduced that DNA methylation may negatively regulate lipid metabolic genes and transcription factors to subtly affect oil accumulation divergence between wild and cultivated peanuts. Our work provided the first glimpse on the regulatory mechanism of gene expression altering for oil accumulation in wild peanut and gene resources for future breeding applications.

Item Type: Article
Divisions: Center of Excellence in Genomics and Systems Biology
Uncontrolled Keywords: Arachis monticola, Arachis hypogaea, oil biosynthesis, expression profiling, DNA methylation, functional genomics
Subjects: Mandate crops > Chickpea
Others > Genetics and Genomics
Depositing User: Mr Nagaraju T
Date Deposited: 08 Aug 2023 10:42
Last Modified: 08 Aug 2023 10:42
Official URL:
Projects: Science and technology innovation Project
Funders: National Natural Science Foundation of China, National Peanut Industry Technology System Construction, National Crop Germplasm Resources Center, National Program for Crop Germplasm Protection of China, Central Public interest Scientific Institution Basal Research Fund, Chinese Academy of Agricultural Sciences
Acknowledgement: The authors thank Dr. Xin Wang from OCRI of CAAS for kindly providing technical guidance in genetic transform of Arabidopsis (Col-0).
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