Recent advancements in CRISPR/Cas technology for accelerated crop improvement

Das, D and Singha, D L and Paswan, R R and Chowdhury, N and Sharma, M and Reddy, P S and Chikkaputtaiah, C (2022) Recent advancements in CRISPR/Cas technology for accelerated crop improvement. Planta (TSI), 255 (5). pp. 1-27. ISSN 0032-0935

[img] PDF - Published Version
Download (1MB)


The likelihood of reduced agricultural production due to highly turbulent climatic conditions increases as the global population expands. The second paradigm of stress-resilient crops with enhanced tolerance and increased productivity against various stresses is paramount to support global production and consumption equilibrium. Although traditional breeding approaches have substantially increased crop production and yield, effective strategies are anticipated to restore crop productivity even further in meeting the world’s increasing food demands. CRISPR/Cas, which originated in prokaryotes, has surfaced as a coveted genome editing tool in recent decades, reshaping plant molecular biology in unprecedented ways and paving the way for engineering stress-tolerant crops. CRISPR/Cas is distinguished by its efficiency, high target specificity, and modularity, enables precise genetic modification of crop plants, allowing for the creation of allelic variations in the germplasm and the development of novel and more productive agricultural practices. Additionally, a slew of advanced biotechnologies premised on the CRISPR/Cas methodologies have augmented fundamental research and plant synthetic biology toolkits. Here, we describe gene editing tools, including CRISPR/Cas and its imitative tools, such as base and prime editing, multiplex genome editing, chromosome engineering followed by their implications in crop genetic improvement. Further, we comprehensively discuss the latest developments of CRISPR/Cas technology including CRISPR-mediated gene drive, tissue-specific genome editing, dCas9 mediated epigenetic modification and programmed self-elimination of transgenes in plants. Finally, we highlight the applicability and scope of advanced CRISPR-based techniques in crop genetic improvement.

Item Type: Article
Divisions: Global Research Program - Accelerated Crop Improvement
Uncontrolled Keywords: Base editing, CRISPR/Cas, CRISPR-TSKO, Crop improvement, Gene drive, Prime editing, Transgene free
Subjects: Others > Crop Improvement
Others > Genetic Engineering
Others > Genetics and Genomics
Depositing User: Mr Arun S
Date Deposited: 04 May 2022 05:01
Last Modified: 04 May 2022 05:01
Official URL:
Acknowledgement: The authors thank the Director, CSIR-NEIST, Jorhat for providing the facilities. CC acknowledges the Council of Scientific and Industrial Research (CSIR), Government of India for financial assistance through Focused Basic Research grant (MLP-0007). This study was partially supported by Science and Engineering Research Board, Department of Science and Technology, Government of India for the core research grant (CRG) File No. CRG/2019/004305
View Statistics

Actions (login required)

View Item View Item