Combining High Oleic Acid Trait and Resistance to Late Leaf Spot and Rust Diseases in Groundnut (Arachis hypogaea L.)

Deshmukh, D B and Marathi, B and Sudini, H K and Variath, M T and Chaudhari, S and Manohar, S S and Rani, C V D and Pandey, M K and Janila, P (2020) Combining High Oleic Acid Trait and Resistance to Late Leaf Spot and Rust Diseases in Groundnut (Arachis hypogaea L.). Frontiers in Genetics (TSI), 11 (514). pp. 1-15. ISSN 1664-8021

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High oleic trait, resistance to rust and late leaf spot (LLS) are important breeding objectives in groundnut. Rust and LLS cause significant economic loss, and high oleic trait is an industry preferred trait that enhances economic returns. This study reports marker-assisted selection to introgress high oleic content, resistance to LLS and rust into Kadiri 6 (K 6), a popular cultivar. The alleles for target traits were selected using linked allele-specific, simple sequence repeats and single nucleotide polymorphic markers. The F1s (384), intercrossed F1s (441), BC1F1s (380), BC1F2s (195), and BC1F3s (343) were genotyped to obtain desired allelic combination. Sixteen plants were identified with homozygous high oleic, LLS and rust resistance alleles in BC1F2, which were advanced to BC1F3 and evaluated for disease resistance, yield governing and nutritional quality traits. Phenotyping with Near-Infrared Reflectance Spectroscopy identified three lines (BC1F3-76, BC1F3-278, and BC1F3-296) with >80% oleic acid. The identified lines exhibit high levels of resistance to LLS and rust diseases (score of 3.0–4.0) with preferred pod and kernel features. The selected lines are under yield testing trials in multi-locations for release and commercialization. The lines reported here demonstrated combining high oleic trait with resistance to LLS and rust diseases.

Item Type: Article
Divisions: Research Program : Asia
Research Program : Genetic Gains
CRP: CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
Uncontrolled Keywords: Groundnut, Marker-assisted backcrossing,High oleic acid, Late leaf spot, Rust, Disease resistance
Subjects: Others > Plant Breeding
Mandate crops > Groundnut
Others > Genetics and Genomics
Others > Plant Disease
Depositing User: Mr Arun S
Date Deposited: 29 Jul 2020 12:58
Last Modified: 03 Sep 2020 03:52
Official URL:
Funders: This work is supported by the OFID, Department of Agriculture and Co-operation (DOAC), Ministry of Agriculture, Government of India, and CGIAR Research Program on Grain Legumes and Dry Land Cereals (CRP-GLDC)
Acknowledgement: Authors are thankful to Mr. Papaiah V., Ms. Chandrakala, Ms. Aparna, Mr. Ranjeet, Mr. Abhishek for the technical assistance during the hybridization. Dr. Y. Shasidhar and Mr. S. Gangurde for providing assistance in genotyping. Ms. Mangala is acknowledged for extending support during disease screening. Authors also acknowledge the OPEC Fund for International Development (OFID) for the fellowship to Dnyaneshwar during his doctoral research.
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