Molecular and phenotypic diversity among chickpea (Cicer arietinum) genotypes as a function of drought tolerance

Sachdeva, S and Bharadwaj, C and Sharma, V and Patil, B S and Soren, K R and Roorkiwal, M and Varshney, R K and Bhat, K V (2018) Molecular and phenotypic diversity among chickpea (Cicer arietinum) genotypes as a function of drought tolerance. Crop and Pasture Science, 69 (2). pp. 142-153. ISSN 1836-0947

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Diversity as a function of drought tolerance may be identified by morphological characters, and molecular tools used to find the most divergent genotypes for breeding programs for drought tolerance in future. The narrow genetic base of chickpea can be circumvented by using diverse lines in breeding programs. Forty chickpea genotypes were studied for their morphological and molecular diversity with an objective of identifying the most diverse drought-tolerant lines. In total, 90 alleles were detected with 3.6 alleles per locus. Polymorphism information content (PIC) values ranged from 0.155 to 0.782 with an average value of 0.4374 per locus. The size of amplified products ranged from 160 bp to 390 bp. Primer TA136 with eight alleles showed the highest PIC value of 0.7825, indicating its ability to differentiate the genotypes at molecular level. DARwin neighbour-joining tree analysis based on dissimilarity estimates was done for the molecular data and sequential agglomerative hierarchical non-overlapping (SAHN) grouping for the morphological data. It could clearly discriminate the tolerance and the sensitivity of genotypes. Two-dimensional principal coordinates analysis (PCoA) plot indicated good diversity for drought tolerance. The genetic similarity coefficients ranged from 0.115 (genotypes BGD72 to ICCV 5308) to 0.828 (genotypes ICCV 10316 to ICCV 92337).

Item Type: Article
Divisions: Research Program : Genetic Gains
Uncontrolled Keywords: genetic diversity, membrane stability index, molecular markers, relative water content, drought tolerance
Subjects: Others > Abiotic Stress
Others > Drought Tolerance
Others > Molecular Biology
Mandate crops > Chickpea
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 03 Mar 2018 15:53
Last Modified: 03 Jul 2018 09:22
Official URL:
Acknowledgement: UNSPECIFIED
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