Expression Analysis of Novel microRNAs in Rice During High Temperature Stress

Sailaja, B and Mangrauthia, S K and Voleti, S R and Subrahmanyam, D and Brajendra, - and Ravindra Babu, V (2017) Expression Analysis of Novel microRNAs in Rice During High Temperature Stress. Bulletin of Environment, Pharmacology and Life Sciences, 6 (1). pp. 225-229. ISSN 2277-1808

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MicroRNAs (miRNAs) are small non-coding RNAs which play an important role in regulating the genes involved in plant growth and development. Several studies showed that miRNAs are involved in plants response to different kinds of abiotic stresses also. In our previous study, temperature responsive miRNAs were predicted in O.sativa. 27 miRNAs were predicted to be novel in rice using homology search. In continuation to our previous study, expression of 14 novel miRNAs was done in shoot and root of 13 days old seedlings of five different rice cultivars using real time PCR. Expression these miRNAs was analyzed in control and high temperature stress environment. Out of 14 predicted novel miRNAs, two novel miRNAs- miR157a and miR165a showed expression in all five rice cultivars. Interestingly, miR165a showed a differential expression pattern among heat tolerant (N22, IR64 and Rasi) and susceptible (Vandana and Sampada) cultivars suggesting that it might have specific role in high temperature tolerance.

Item Type: Article
Uncontrolled Keywords: Expression Analysis, Novel microRNAs, Rice, High Temperature Stress, Abiotic stress, N22, genes
Subjects: Others > Abiotic Stress
Others > Rice
Others > Genetics and Genomics
Others > Climate Change
Depositing User: Mr Ramesh K
Date Deposited: 03 Mar 2018 10:25
Last Modified: 03 Mar 2018 10:26
Official URL:
Acknowledgement: Authors are highly thankful to the Director, Indian Institute of Rice Research, for his kind support. Financial support received from NICRA (National Initiative of Climate and Resilient Agriculture) project is acknowledged.
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