Fine mapping studies identified a 113 kb region within “QTL-hotspot_a” for seed weight and drought related traits in chickpea

Kale, S M and Jaganathan, D and Roorkiwal, M and Thudi, M and Purushothaman, R and Praveenkumar, M and Krishnamurthy, L and Kavi Kishor, P B and Gaur, P M and Varshney, R K (2017) Fine mapping studies identified a 113 kb region within “QTL-hotspot_a” for seed weight and drought related traits in chickpea. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.

PDF - Published Version
Download (127kB) | Preview


A “QTL-hotspot” region of 7 Mb size for drought component traits was identified on CaLG04, using a recombinant inbred line (RIL) population (ICC 4958 × ICC 1882) in chickpea. Further, skimbased genotyping by sequencing (GBS) approach with large SNP markers delimited the “QTL-hotspot” region into two sub-regions; viz, “QTL-hotspot_a” of 139.22 kb and “QTL-hotspot_b” of 153.36 kb, on the kabuli draft genome sequence. In order to validate and identify more recombinations in the sub-regions for further refinement, a fine mapping population with 1,911 lines was developed. Flanking markers of the two “QTL-hotspot” sub-regions were converted to KASPar assays and used to screen the fine mapping population consisting of 1,911 lines. As a result, 19 F2:3 recombinant families were identified. These families were phenotyped for seed weight and other drought-related traits. Comparison of genotype and phenotype data identified a genomic region of ~113 Kb size within “QTL-hotspot_a” responsible for 100 seed weight (100SDW) and other drought-related traits in chickpea. Subsequently, a syntenic study between the refined “QTL-hotpsot” region and desi genome identified a 2 Mb region on Ca_LG_4 pseudomolecule. Whole genome re-sequencing (WGRS) analysis of selected lines from each recombinant family identified several non-synonymous and InDel mutations within important candidate genes. Functional validation of these genes will help decipher the mechanism of drought stress tolerance in chickpea.

Item Type: Conference or Workshop Item (Poster)
Divisions: Research Program : Genetic Gains
Uncontrolled Keywords: Fine mapping studies, QTL-hotspot, Drought related traits, Chickpea
Subjects: Others > Abiotic Stress
Others > Drought Tolerance
Mandate crops > Chickpea
Others > Drought
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 22 Nov 2017 05:40
Last Modified: 03 Jul 2018 09:05
Acknowledgement: UNSPECIFIED
View Statistics

Actions (login required)

View Item View Item