Molecular Mapping of Oil Content and Fatty Acids Using Dense Genetic Maps in Groundnut (Arachis hypogaea L.)

Shasidhar, Y and Vishwakarma, M K and Pandey, M K and Janila, P and Variath, M T and Manohar, S S and Nigam, S N and Guo, B and Varshney, R K (2017) Molecular Mapping of Oil Content and Fatty Acids Using Dense Genetic Maps in Groundnut (Arachis hypogaea L.). Frontiers in Plant Science, 8 (794). pp. 1-14. ISSN 1664-462X

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Enhancing seed oil content with desirable fatty acid composition is one of the most important objectives of groundnut breeding programs globally. Genomics-assisted breeding facilitates combining multiple traits faster, however, requires linked markers. In this context, we have developed two different F2 mapping populations, one for oil content (OC-population, ICGV 07368 × ICGV 06420) and another for fatty acid composition (FA-population, ICGV 06420 × SunOleic 95R). These two populations were phenotyped for respective traits and genotyped using Diversity Array Technology (DArT) and DArTseq genotyping platforms. Two genetic maps were developed with 854 (OC-population) and 1,435 (FA-population) marker loci with total map distance of 3,526 and 1,869 cM, respectively. Quantitative trait locus (QTL) analysis using genotyping and phenotyping data identified eight QTLs for oil content including two major QTLs, qOc-A10 and qOc-A02, with 22.11 and 10.37% phenotypic variance explained (PVE), respectively. For seven different fatty acids, a total of 21 QTLs with 7.6–78.6% PVE were identified and 20 of these QTLs were of major effect. Two mutant alleles, ahFAD2B and ahFAD2A, also had 18.44 and 10.78% PVE for palmitic acid, in addition to oleic (33.8 and 17.4% PVE) and linoleic (41.0 and 19.5% PVE) acids. Furthermore, four QTL clusters harboring more than three QTLs for fatty acids were identified on the three LGs. The QTLs identified in this study could be further dissected for candidate gene discovery and development of diagnostic markers for breeding improved groundnut varieties with high oil content and desirable oil quality.

Item Type: Article
Divisions: Research Program : Genetic Gains
Research Program : Asia
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Molecular Mapping, Genetic Maps, Groundnut, DArT/DArTseq, genetic map, QTLs, oil content, fatty acids, peanut
Subjects: Others > Molecular Biology
Mandate crops > Groundnut
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 23 May 2017 10:40
Last Modified: 05 Sep 2017 05:19
Official URL:
Acknowledgement: The work presented in this article is the contribution from research projects sponsored by Department of Agriculture and Co-operation (DAC), Ministry of Agriculture, Government of India under Integrated Scheme of Oilseeds, Pulses, Oilpalm and Maize (ISOPOM) program. YS acknowledges the award of Junior Research Fellowship from Department of Biotechnology, Government of India. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium.
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