Comparative transcriptome analysis of aerial and subterranean pods development provides insights into seed abortion in peanut

Zhu, W and Chen, X and Li, H and Zhu, F and Hong, Y and Varshney, R K and Liang, X (2014) Comparative transcriptome analysis of aerial and subterranean pods development provides insights into seed abortion in peanut. Plant Molecular Biology, 85 (4-5). pp. 395-409. ISSN 1573-5028

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The peanut is a special plant for its aerial flowering but subterranean fructification. The failure of peg penetration into the soil leads to form aerial pod and finally seed abortion. However, the mechanism of seed abortion during aerial pod development remains obscure. Here, a comparative transcriptome analysis between aerial and subterranean pods at different developmental stages was produced using a customized NimbleGen microarray representing 36,158 unigenes. By comparing 4 consecutive time-points, totally 6,203 differentially expressed genes, 4,732 stage-specific expressed genes and 2,401 specific expressed genes only in aerial or subterranean pods were identified in this study. Functional annotation showed their mainly involvement in biosynthesis, metabolism, transcription regulation, transporting, stress response, photosynthesis, signal transduction, cell division, apoptosis, embryonic development, hormone response and light signaling, etc. Emphasis was focused on hormone response, cell apoptosis, embryonic development and light signaling relative genes. These genes might function as potential candidates to provide insights into seed abortion during aerial pod development. Ten candidate genes were validated by Real-time RT-PCR. Additionally, consistent with up-regulation of auxin response relative genes in aerial pods, endogenous IAA content was also significantly increased by HPLC analysis. This study will further provide new molecular insight that auxin and auxin response genes potentially contribute to peanut seed and pod development.

Item Type: Article
Divisions: RP-Grain Legumes
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Aerial pod, Subterranean pod, Transcriptome, Peanut, Seed abortion, Development
Subjects: Mandate crops > Groundnut
Others > Genetics and Genomics
Depositing User: Mr Siva Shankar
Date Deposited: 05 May 2014 06:18
Last Modified: 11 Jun 2014 03:56
Official URL:
Funders: National Natural Science Foundation(China), Natural Science Foundation(Guangdong Province), Science and Technology Planning Project(Guangdong Province), Pearl River Science and Technology Nova(Guangzhou), Modern Agro-industry Technology Research System
Acknowledgement: This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
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