Complex and shifting interactions of phytochromes regulate fruit development in tomato

Gupta, Suresh Kumar and Sharma, S and Santisree, P and Kilambi, H V and Appenroth, K and Sreelakshmi, Y and Sharma, R (2013) Complex and shifting interactions of phytochromes regulate fruit development in tomato. Plant, Cell & Environment. pp. 1-57. ISSN 1365-3040

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Tomato fruit ripening is a complex metabolic process regulated by a genetical hierarchy. A subset of this process is also modulated by light-signaling, as mutants encoding negative regulators of phytochrome signal transduction, show higher accumulation of carotenoids. In tomato phytochromes are encoded by a multi-gene family, namely PhyA, PhyB1, PhyB2, PhyE and PhyF, however, their contribution to fruit development and ripening has not been examined. Using single phytochrome mutants- phyA, phyB1 and phyB2 and multiple mutants- phyAB1, phyB1B2 and phyAB1B2, we compared the on-vine transitory phases of ripening till fruit abscission. The phyAB1B2 mutant showed accelerated transitions during ripening with shortest time to fruit abscission. Comparison of transition intervals in mutants indicated a phase-specific influence of different phytochrome species either singly or in combination on the ripening process. Examination of off-vine ripened fruits indicated that ripening specific carotenoid accumulation was not obligatorily dependent on light and even dark incubated fruits accumulated carotenoids. The accumulation of transcripts and carotenoids in off-vine and on-vine ripened mutant fruits indicated a complex and shifting phase-dependent modulation by phytochromes(s). Our results indicate that in addition to regulating carotenoid levels in tomato fruits, phytochrome(s) also regulate the time required for phase transitions during ripening.

Item Type: Article
Uncontrolled Keywords: Tomato; photoreceptors; phytochrome; fruit ripening; carotenoids
Subjects: Others > Agriculture-Farming, Production, Technology, Economics
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 27 Jan 2014 06:19
Last Modified: 21 Aug 2018 04:56
Official URL:
Funders: Government of India - Department of Biotechnology
Acknowledgement: This work was supported by DBT, New Delhi (BT/PR10903/GBD/27/123/2008 to Y Sreelakshmi; BT/PR11671/PBD/16/828/2008, BT/PR/6803/PBD/16/621/2005 and BT/PR/5275/AGR-/16/465/2004 to R Sharma and Y Sreelakshmi; BR/PR/4543/AGR/16/372/2003 to R Sharma); IAEA, Vienna (15632/R0 to R Sharma; 15166/R0 to Y Sreelakshmi); Research fellowship from UGC, New Delhi, (HV Kilambi, P Santisree) and from CSIR, New Delhi (SK Gupta).The travel grants from AvH Foundation, Germany to Rameshwar Sharma and Klaus Appenroth to visit respective laboratories is gratefully acknowledged. Klaus Appenroth was Visiting Professor at University of Hyderabad supported by UGC -SAP.
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