Deep sequencing analysis of the transcriptomes of peanut aerial and subterranean young pods identifies candidate genes related to early embryo abortion

Chen, X and Zhu, W and Azam, S and Li, H and Zhu, F and Li, H and Hong, Y and Liu, H and Zhang, E and Wu, H and Yu, S and Zhou, G and Li, S and Zhong, N and Wen, S and Li, X and Knapp, S J and Ozias-Akins, P and Varshney, R K and Liang, X (2013) Deep sequencing analysis of the transcriptomes of peanut aerial and subterranean young pods identifies candidate genes related to early embryo abortion. Plant Biotechnology Journal, 11 (1). pp. 115-127.

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The failure of peg penetration into the soil leads to seed abortion in peanut. Knowledge of genes involved in these processes is comparatively deficient. Here, we used RNA-seq to gain insights into transcriptomes of aerial and subterranean pods. More than 2 million transcript reads with an average length of 396 bp were generated from one aerial (AP) and two subterranean (SP1 and SP2) pod libraries using pyrosequencing technology. After assembly, sets of 49 632, 49 952 and 50 494 from a total of 74 974 transcript assembly contigs (TACs) were identified in AP, SP1 and SP2, respectively. A clear linear relationship in the gene expression level was observed between these data sets. In brief, 2194 differentially expressed TACs with a 99.0% true-positive rate were identified, among which 859 and 1068 TACs were up-regulated in aerial and subterranean pods, respectively. Functional analysis showed that putative function based on similarity with proteins catalogued in UniProt and gene ontology term classification could be determined for 59 342 (79.2%) and 42 955 (57.3%) TACs, respectively. A total of 2968 TACs were mapped to 174 KEGG pathways, of which 168 were shared by aerial and subterranean transcriptomes. TACs involved in photosynthesis were significantly up-regulated and enriched in the aerial pod. In addition, two senescence-associated genes were identified as significantly up-regulated in the aerial pod, which potentially contribute to embryo abortion in aerial pods, and in turn, to cessation of swelling. The data set generated in this study provides evidence for some functional genes as robust candidates underlying aerial and subterranean pod development and contributes to an elucidation of the evolutionary implications resulting from fruit development under light and dark conditions.

Item Type: Article
Uncontrolled Keywords: peanut, aerial and subterranean pod, transcriptome, RNA sequencing.
Subjects: Mandate crops > Groundnut
Others > Genetics and Genomics
Depositing User: Mr Siva Shankar
Date Deposited: 12 Nov 2012 06:27
Last Modified: 18 Apr 2016 09:04
Official URL:
Funders: National Natural Science Foundation of China (No. 30971819 and 30900907), Natural Science Foundation of Guangdong Province (No. 10151064001000002), Science and Technology Planning Project of Guangdong Province (2011B010500019), Pearl River Science and Technology Nova of Guangzhou (No. 2011J2200035), Modern Agro-industry Technology Research System (No. nycycx-19, CARS-14)
Acknowledgement: UNSPECIFIED
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