Protease Inhibitors in Wild Relatives of Pigeonpea against the Cotton Bollworm/Legume Pod Borer, Helicoverpa armigera

Parde, V D and Sharma, H C and Kachole, M S (2012) Protease Inhibitors in Wild Relatives of Pigeonpea against the Cotton Bollworm/Legume Pod Borer, Helicoverpa armigera. American Journal of Plant Sciences, 3. pp. 627-635. ISSN 2158-2750 (In Press)

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Cotton bollworm/legume pod borer, Helicoverpa armigera is one of the most damaging pests worldwide. Because of the difficulties associated with chemical control of this pest, emphasis has been placed on developing transgenic plants with resistance to this pest. Since toxin genes from the bacterium, Bacillus thuringiensis (Bt) have been deployed on a large scale, there is need to scout for alternate genes which could be deployed alone or in combination with the Bt genes for pest management. Therefore, we evaluated the wild relatives of pigeonpea, which have shown high levels of resis-tance to this pest, for the protease inhibitors (PIs) under in vivo and in vitro inhibitions. Accession belonging to Cajanus albicans, C. cajanifolius, C. sericeus, Flemingia bracteata, and Rhynchosia bracteata showed complete inhibition of H. armigera gut proteinases (HaGPs). Some of the C. scarabaeoides accessions (ICPW 116, 152, 278 and 280) exhibited partial inhibition at low concentrations of the PIs. All accessions of wild relatives of pigeonpea showed high to moder-ate level of inhibition at pH 7.8. Cultivated pigeonpea, ICPL 87 exhibited monomorphism in terms of trypsin inhibitor (TI) and chymotrypsin inhibitor (CTI) isoforms, contrary to the diverse inhibitory profiles of wild pigeonpeas. Cajanus albicans, C. platycarpus, C. scarabaeoides, and R. bracteata showed more number of TI and CTI bands than the culti-vated pigeonpea. Protease inhibitor isoforms of wild relatives of pigeonpea showed significant variation in number, band pattern, and protein specificities towards trypsin, chymotrypsin, and H. armigera gut proteinases (HaGPs) as compared to the cultivated pigeonpea. The PIs from the wild relatives of pigeonpea showed considerable potential against the HaGPs, and could be considered as potential candidates for use in genetic transformation of crops for pest management, including H. armigera.

Item Type: Article
Uncontrolled Keywords: Wild Relatives; Pigeonpea; Protease Inhibitors; Helicoverpa armigera; Transgenic Plants; Pest Management
Subjects: Mandate crops > Pigeonpea
Others > Entomology
Depositing User: Mr Siva Shankar
Date Deposited: 16 May 2012 05:49
Last Modified: 01 Feb 2013 04:54
Official URL:
Projects: The Andhra Pradesh-Netherlands Project on Biotechnology
Funders: Department of Agriculture and Cooperation, Govt of India
Acknowledgement: We thank the staff of Entomology, ICRISAT, for their help in maintaining the culture of the pod borer, Heli-coverpa armigera, and for raising the wild relatives of pigeonpea in the field for procuring the seeds for evalua-tion of their proteinase inhibitory activity. We also thank Dr Md Akbar for reviewing the manuscript.
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