Development of a Core RFLP Map in Maize Using an Immortalized F2 Population

Gardiner, J M and Coe, E H and Melia-Hancock, S and Hoisington, D A (1993) Development of a Core RFLP Map in Maize Using an Immortalized F2 Population. Genetics, 134. pp. 917-930. ISSN 1943-2631

PDF - Published Version
Download (1MB) | Preview


A map derived from restriction fragment length polymorphisms (RFLPs)i n maize (Zea mays L.) is presented. The map was constructed in an immortalized Tx303 X C0159 FP mapping population that allowed for an unlimited number of markers to be mapped and pooled Fs seed to be distributed to other laboratories. A total of 215 markers consisting of 159 genomic clones, 16 isozymes and 35 cloned geneso f defined function have been placed on 10 chromosomes. An examination of segregation data has revealed several genomic regions with aberrant segregation ratios favoring either parenot r the heterozygote. Mapping of cloned genes and isozymes that have been previously mapped by functional criteria has provided 29 points of alignment with the classical maize genetic map. Screening of all mapped RFLP probes against a collection of U.S. Corn Belt germplasm using EcoRI, Hind111 and EcoRV has resulted in a set of 97 core markers being defined. The designation of a set of core markers allows the maize genome to be subdivided into a series of bins which serve as the backbone for maize genetic information and database boundariesT. he merits and applicationso f core markers v and bins are discussed.

Item Type: Article
Subjects: Others > Maize
Depositing User: Mr Siva Shankar
Date Deposited: 15 Mar 2012 05:33
Last Modified: 16 Sep 2013 12:34
Official URL:
Acknowledgement: [NOTE: D. A. Hoisington is presently with ICRISAT (Patancheru)]---We would like to acknowledge the excellente chnical assistance of RANDALL GROCAONS, CARH EREDIA andC ASEY HOWELLT. he typing of this manuscript by MARY ANSNT EYAERTis also gratefully acknowledged. We would like to thank BENB URR, J. PG.U STAFSON and SCOTTT INCEfYor comments on the manuscript andw e thank the many colleagues who provided probes and information; especially TIMH ELENTJARIfSo r suggesting the concept ofb ins and providing the PstI library and NPI probes and BEN BURR for providing probes and mapping information. This work was supported by gifts from Pioneer Hi-Bred International, Garst (IC1 Seeds), United Agriseeds (Dow-Elanco), and grant DIR87-21921 from the National Science Foundation.
View Statistics

Actions (login required)

View Item View Item