Biological control of late leaf spot of peanut (Arachis hypogaea) with chitinolytic bacteria

Kishore, G K and Pande, S and Podile, A R (2005) Biological control of late leaf spot of peanut (Arachis hypogaea) with chitinolytic bacteria. Phytopathology , 95 (10). pp. 1157-1165. ISSN 0031-949X

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Late leaf spot (LLS), caused by Phaeoisariopsis personata, is a foliar disease of groundnut or peanut (Arachis hypogaea) with high economic and global importance. Antifungal and chitinolytic Bacillus circulans GRS 243 and Serratia marcescens GPS 5, selected among a collection of 393 peanut-associated bacteria, were applied as a prophylactic foliar spray and tested for control of LLS. Chitin-supplemented application of B. circulans GRS 243 and S. marcescens GPS 5 resulted in improved biological control of LLS disease. Supplementation of bacterial cells with 1% (wt/vol) colloidal chitin reduced lesion frequency by 60% compared with application of bacterial cells alone, in the greenhouse. Chitin-supplemented application of GRS 243 and GPS 5 also resulted in improved and stable control of LLS in a repeated field experiment and increased the pod yields by 62 and 75%, respectively, compared with the control. Chitin-supplemented application of GPS 5 was tested in six on-farm trials, and the increase in pod yields was up to 48% in kharif (rainy season). A 55-kDa chitinase was purified from the cell-free culture filtrate of GPS 5 by affinity chromatography and gel filtration. Purified chitinase of S. marcescens GPS 5 (specific activity 120 units) inhibited the in vitro germination of P. personata conidia, lysed the conidia, and effectively controlled LLS in greenhouse tests, indicating the importance of chitinolysis in biological control of LLS disease by GPS 5

Item Type: Article
Uncontrolled Keywords: Biotrophic, phylloplane, rhizobacteria
Subjects: Mandate crops > Groundnut
Depositing User: Ms K Syamalamba
Date Deposited: 17 Nov 2011 03:34
Last Modified: 17 Nov 2011 03:34
Official URL:
Funders: Andhra Pradesh-Netherlands Biotechnology Programme
Acknowledgement: The Andhra Pradesh-Netherlands Biotechnology Programme supported this work in the form of a research grant to A. R. Podile and senior research fellowship to G. K. Kishore. We thank the UGC-SAP and DSTFIST programmes of the Department of Plant Sciences, University of Hyderabad
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