Embryo Rescue in Wide Crosses in Arachis. 2. Embryo Development in Cultured Peg Tips of Arachis hypogaea

Pattee, H E and Stalker, H T and Moss, J P (1987) Embryo Rescue in Wide Crosses in Arachis. 2. Embryo Development in Cultured Peg Tips of Arachis hypogaea. Annals of Botany, 61 (1). pp. 103-112.

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Embryo rescue techniques in Arachis are potentially important for recovering interspecific hybrids which have the propensity to abort. Pegs are commonly produced in interspecific crosses, but either they fail to reach the soil because growth is arrested, or pods are produced but embryo development is never re-initiated. Peg tips, with the ovule and embryo, of A. hypogaea L. cv. ‘NC 6’, were used to determine whether peg tips can be used as nurse tissue for in vitro culture of embryos. Tissues were collected 1, 2, 3 and 4 d after self-pollination, after which peg meristems were removed from half the pegs, and cultured on five media combinations. Continued reproductive development was observed for embryos cultured at all four collection days; however, the highest frequency of growth was observed in 1-d-old tissues. Evidence is presented that meristematic activity may restrict embryo growth in the 2- to 4-d-old embryos and, once the sequence of events is initiated to slow embryo growth, it is not easily reversed in vitro. Achievements of embryo growth to multicellular, globular stages (stages 1–1 or 1–2) encourage the development of methods to recover very young embryos through tissue-culture techniques.

Item Type: Article
Subjects: Others
Depositing User: Mr Charan Sai Ch
Date Deposited: 06 Nov 2011 02:52
Last Modified: 06 Nov 2011 02:52
URI: http://oar.icrisat.org/id/eprint/3707
Official URL: http://aob.oxfordjournals.org/content/61/1/103.abs...
Acknowledgement: UNSPECIFIED
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