An RFLP-based genetic map of pearl millet (Pennisetum glaucum)

Liu, C J and Witcombe, J R and Pittaway, T S and Nash, M and Hash, C T and Busso, C S and Gale, M D (1994) An RFLP-based genetic map of pearl millet (Pennisetum glaucum). Theoretical and Applied Genetics, 89 (4). pp. 481-487. ISSN 0040-5752

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Analysis of a sample of diverse pearl millet genotypes with 200 genomic DNA probes revealed this crop species to be extremely polymorphic. Among these genotypes, 85% of probes detected polymorphism using only two restriction enzymes, with an average pair-wise polymorphism between all of the probe-enzyme combinations of 56%. Two crosses were employed to construct an RFLP-based genetic map. In an intervarietal F2 population, derived from a single F1 plant, 181 loci were placed on a linkage map. The total length of this map, which comprised seven linkage groups, was 303 cM and the average map distance between loci was about 2 cM, although a few intervals in excess of 10 cM were present at the ends of a few linkage groups. Very few clones, including those which hybridized to more than one copy, detected more than one locus in the pearl millet genome. The analysis was complicated initially because 83 of the 181 loci mapped to a single linkage group. Analysis of a second cross identified a probable translocation breakpoint in the middle of this large linkage group.

Item Type: Article
Subjects: Mandate crops > Millets
Depositing User: Mr Charan Sai Ch
Date Deposited: 06 Dec 2011 15:13
Last Modified: 06 Dec 2011 15:13
Official URL:
Acknowledgement: UNSPECIFIED
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