Chickpea chlorotic dwarf virus, a new leafhopper-transmitted geminivirus of chickpea in India

Horn, N M and Reddy, S V and Roberts, I M and Reddy, D V R (1993) Chickpea chlorotic dwarf virus, a new leafhopper-transmitted geminivirus of chickpea in India. Annals of Applied Biology, 122 (3). pp. 467-479. ISSN 1744-7348

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A disease of chickpea in India, characterised by chlorosis, severe stunting and phloem browning, was shown to be caused by a geminivirus. This virus was transmitted by the leafhopper Orosius orientalis from chickpea to chickpea and several other plant species. A method for purification of this virus was devised and a polyclonal antiserum produced. The majority of the purified particles were geminate. The size of the coat protein was shown to be 32 kD and the nucleic acid was shown to be circular ssDNA of 2900 nucleotides. By immunosorbent electron microscopy this virus was shown to be unrelated to the leafhopper-transmitted geminiviruses known to infect dicotyledons such as beet curly top, bean summer death and tobacco yellow dwarf viruses. On the basis of particle morphology, leafhopper transmission, host range and serology this virus was considered to be a new, hitherto undescribed, geminivirus and was named chickpea chlorotic dwarf virus.

Item Type: Article
Uncontrolled Keywords: eminivirus;chickpea stunt;chickpea chlorotic dwarf virus;leafhopper;India;Orosius orientalis;bean summer death virus;tobacco yellow dwarf virus
Subjects: Mandate crops > Chickpea
Depositing User: Mr Charan Sai Ch
Date Deposited: 23 Oct 2011 10:36
Last Modified: 23 Oct 2011 10:36
Official URL:
Funders: Government of Netherland- Ministry of Foreign Affairs-DGIS, International Crops Research Institute for the Semi-Arid Tropics
Acknowledgement: The senlor author is greatly indebted to the Directorate General for Development Cooperation (DGIS) of the Netherlands Mlni5try of Foreign Affairs and to the Director General of IC'RISAT for providing financial support during his per~od of research at ICRISAT. We would like to thank Drs Y. L. Nene and D. McDonald for their valuable comments and $upport. We are grateful to Dr P. Narayana Swamy of the Tamil Nadu Agricultural University, Coimbatore for providing us with a culture of Oroslus orientalis and to Dr Viraktamath, University of Agricultural Sciences, Bangalore, and the International Institute of Entomology, CAB International, UK, for confirming its identity. We would like to thank Drs K. R. Bock, J . E. Duffus, R. 1. B. Francki, B. D. Harrison, K. Lindsten, D. L. Mumford and J. E. Thomas for their generous gifts of antisera and Dr J . Stanley (John lnnes Institute, UK) for providing the results of hybridisation with BCTV DNA, Dr D. J. Robinson and G. H. Duncan (SCRI, UK) for providing the electron micrograph of the viral DNA and to Mr A. K. Murthy (ICRISAT) for help with the electron microscopy.
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