Zhang, X and Pandey, M K and Wang, J and Zhao, K and Ma, X and Li, Z and Zhao, K and Gong, F and Guo, B and Varshney, R K and Yin, D (2021) Chromatin spatial organization of wild type and mutant peanuts reveals high-resolution genomic architecture and interaction alterations. Genome Biology, 22. pp. 1-21. ISSN 1474-760X
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Abstract
Background Three-dimensional (3D) chromatin organization provides a critical foundation to investigate gene expression regulation and cellular homeostasis. Results Here, we present the first 3D genome architecture maps in wild type and mutant allotetraploid peanut lines, which illustrate A/B compartments, topologically associated domains (TADs), and widespread chromatin interactions. Most peanut chromosomal arms (52.3%) have active regions (A compartments) with relatively high gene density and high transcriptional levels. About 2.0% of chromosomal regions switch from inactive to active (B-to-A) in the mutant line, harboring 58 differentially expressed genes enriched in flavonoid biosynthesis and circadian rhythm functions. The mutant peanut line shows a higher number of genome-wide cis-interactions than its wild-type. The present study reveals a new TAD in the mutant line that generates different chromatin loops and harbors a specific upstream AP2EREBP-binding motif which might upregulate the expression of the GA2ox gene and decrease active gibberellin (GA) content, presumably making the mutant plant dwarf. Conclusions Our findings will shed new light on the relationship between 3D chromatin architecture and transcriptional regulation in plants.
| Item Type: | Article |
|---|---|
| Divisions: | Center of Excellence in Genomics and Systems Biology |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | Peanut, 3D structure, Hi-C, ATAC-seq, Gene expression, Gene regulation |
| Subjects: | Mandate crops > Groundnut Others > Genetics and Genomics |
| Depositing User: | Mr Nagaraju T |
| Date Deposited: | 04 Jun 2025 05:01 |
| Last Modified: | 04 Jun 2025 05:01 |
| URI: | http://oar.icrisat.org/id/eprint/13113 |
| Official URL: | https://link.springer.com/article/10.1186/s13059-0... |
| Projects: | Key Scientific and Technological Project in Henan Province |
| Funders: | National Natural Science Foundation of China, Key Program of NSFC-Henan United Fund |
| Acknowledgement: | The authors are grateful to the anonymous reviewers for their helpful suggestions about the manuscript. The work reported in this article was undertaken as a part of the CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC). ICRISAT is a member of the CGIAR. |
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