Effects of Plastic Film Mulching on Soil Enzyme Activities and Stoichiometry in Dryland Agroecosystems

Liu, M and Zhao, X and Hossain, M E and Wang, S and Dong, W and Gopalakrishnan, S and Liu, E (2022) Effects of Plastic Film Mulching on Soil Enzyme Activities and Stoichiometry in Dryland Agroecosystems. Plants (TSI), 11(13) (1748). pp. 1-14. ISSN 2223-7747

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Soil extracellular enzymes are pivotal for microbial nutrient cycling in the ecosystem. In order to study the effects of different nitrogen application rates under plastic film mulching on soil extracellular enzyme activities and stoichiometry, five nitrogen application levels (i.e., 0, 90, 150, 225 and 300 kg·hm−2) were set based on two treatments: plastic film mulching (PM) and no film mulching (LD). We measured the soil extracellular enzyme activities (EEAs) and stoichiometry (EES) of four en-zymes (i.e., β-1,4-glucosidase (βG), leucine aminopeptidase (LAP), β-1,4-N-acetylaminoglucosidase (NAG) and alkaline phosphatase (AP)) involved in the C, N and P cycles of soil microorganisms in surface soil at five maize growth stages (seedling stage, jointing stage, trumpet stage, grout stage and harvest stage). The results showed that there were significant differences in soil EEA at different maize growth stages. The soil nutrient content and soil EEA were significantly improved under PM, and the stoichiometric ratio of extracellular enzymes (EC:N:P) was closer to 1:1:1, which indicated that PM was beneficial to the balance of soil nutrients and the activity of microorganisms. At each stage, with the increase in nitrogen application levels, the soil EEA showed a trend of increasing first and then decreasing (or remained unchanged), and both LD and PM treatments reached their highest activity at the 225 kg·hm−2 nitrogen application rate. When the nitrogen application level was less than 225 kg·hm−2, the soil enzyme activity was mainly limited by the N nutrient, and when the nitrogen application level reached 300 kg·hm−2, it was mainly limited by the P nutrient. RDA and correlation analysis showed that the soil C:P, C:N, N:P and pH had significant effects on soil βG, NAG + LAP and AP activities as well as EC:N, EC:P and EN:P.

Item Type: Article
Divisions: Others
Uncontrolled Keywords: Nitrogen levels, Maize of stage, Enzyme activity, Enzyme stoichiometric ratio, Nutrient limitation
Subjects: Others > Soil
Others > Drylands
Depositing User: Mr Ramesh MNR
Date Deposited: 12 Sep 2022 07:44
Last Modified: 13 Sep 2022 07:58
URI: http://oar.icrisat.org/id/eprint/12006
Official URL: https://doi.org/10.3390/plants11131748
Funders: This work was supported by the National Natural Science Foundation of China (grant no. 31961143017 and 31470556), the National Key R&D Program of China (2021YFE0101300) and the Bio-Water Saving and Dry Farming Innovation Team Project of CAAS.
Acknowledgement: We express our sincere gratitude to the Shouyang Key Dry Farming Field Observation and Experimental Station of the Ministry of Agriculture and Rural Affairs for providing the research platform.
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