SSR markers associated with resistance to rust (Puccinia arachidis Speg.) in groundnut (Arachis hypogaea L.)

Varma, T S N and Dwivedi, S L and Pande, S and Gowda, M V C (2005) SSR markers associated with resistance to rust (Puccinia arachidis Speg.) in groundnut (Arachis hypogaea L.). SABRAO Journal of Breeding and Genetics, 37 (2). pp. 107-119.

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Rust (Puccinia arachidis Speg.) is one of the important foliar diseases that causes substantial losses to groundnut (Arachis hypogaea L.) production and reduces seed quality. Resistance to rust is associated with many undesirable pod and seed characteristics. It is reported to be controlled either by few recessive genes or is inherited as a quantitative trait. DNA marker technology is used to minimize linkage drag and hasten the development of new resistant varieties. This experiment was initiated to study molecular variation among parents and identify SSR markers associated with rust resistance in groundnut. The parents and F2 populations of the two crosses were evaluated for resistance to rust under greenhouse conditions. None of the SSR primer pairs showed intra-accession variability among parents. Seven and eight primer pairs detected polymorphic variation between ICGV 99003 and TMV 2, and ICGV 99005 and TMV 2, respectively. They either belonged to 'ATT' or 'AG' SSR repeat families. Wilcoxon test was used to test the significance of association between marker and rust disease score. Rust resistance was associated with two SSR alleles (pPGPseq3A1271 and pPGPseq3A1390) in ICGV 99003 × TMV 2 and seven SSR alleles (pPGPseq5D5270, pPGPseq5D5295, pPGPseq5D5325, pPGPseq16F1315, pPGPseq16F1424, pPGPseq7F6128, and pPGPseq13A7292) in ICGV 99005 × TMV 2. SSR markers associated with rust resistance should facilitate the rapid identification and transfer of chromosomal region(s) into elite breeding lines by using marker-assisted backcross breeding in groundnut.

Item Type: Article
Subjects: Mandate crops > Groundnut
Depositing User: Library ICRISAT
Date Deposited: 11 Sep 2011 06:07
Last Modified: 11 Nov 2011 07:42
Official URL:
Acknowledgement: UNSPECIFIED
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