A systematic dissection of the mechanisms underlying the natural variation of silique number in rapeseed ( Brassica napus L.) germplasm

Li, S and Zhu, Y and Varshney, R K and Zhan, J and Zheng, X and Shi, J and Wang, X and Liu, G and Wang, H (2020) A systematic dissection of the mechanisms underlying the natural variation of silique number in rapeseed ( Brassica napus L.) germplasm. Plant Biotechnology Journal (TSI), 18 (2). pp. 568-580. ISSN 1467-7644

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Silique number is the most important component of yield in rapeseed (Brassica napusL.). Todissect the mechanism underlying the natural variation of silique number in rapeseedgermplasm, a series of studies were performed. A panel of 331 core lines was employed togenome-wide association study (GWAS), and 27 loci (including 20 novel loci) were identified.The silique number difference between the more- and fewer-silique lines can be attributed tothe accumulative differences in flower number and silique setting rate. Each of themaccounted for 75.2% and 24.8%, respectively. The silique number was highly associated withthe total photosynthesis and biomass. Microscopic analysis showed that the differencebetween extremely more- and fewer-silique lines normally occurred at the amount of flowerbud but not morphology. Transcriptome analysis of shoot apical meristem (SAM) suggestedthat most of enriched groups were associated with the auxin biosynthesis/metabolism,vegetative growth and nutrition/energy accumulation. By integrating GWAS and RNA-seqresults, six promising candidate genes were identified, and some of them were related tobiomass accumulation. In conclusion, the natural variation of silique number is largely affectedby the biomass and nutrition accumulation, which essentially reflects the positive regulatoryrelationship between the source and sink. Our study provides a comprehensive and systematicexplanation for natural variation of silique number in rapeseed, which provides a foundationfor its improvement.

Item Type: Article
Divisions: Research Program : Genetic Gains
Uncontrolled Keywords: Brassica napusL., Siliquenumber, GWAS, RNA-seq, Leaf area, Photosynthesis
Subjects: Others > Genetics and Genomics
Others > Germplasm
Depositing User: Mr Arun S
Date Deposited: 17 Mar 2020 05:49
Last Modified: 17 Mar 2020 05:51
URI: http://oar.icrisat.org/id/eprint/11398
Official URL: https://doi.org/10.1111/pbi.13224
Acknowledgement: This research was supported by the National Key Research andDevelopment Program (2016YFD0100305), the National KeyBasic Research Development Program of China (2015CB150203),the Natural Science Foundation of Hubei Province (2018CFA075),the Natural Science Foundation (31101181), the RapeseedIndustry Technology System (CARS-13), the Agricultural Scienceand Technology Innovation Project (CAAS-ASTIP-2013-OCRI), theCore Research Budget of the Non-profit Governmental ResearchInstitution (1610172017001).
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