Antinutritional factors in pearl millet grains: Phytate and goitrogens content variability and molecular characterization of genes involved in their pathways

Boncompagni, E and Orozco-Arroyo, G and Cominelli, E and Gangashetty, P I and Grando, S and Kwaku Zu, T T and Daminati, M G and Nielsen, E and Sparvoli, F (2018) Antinutritional factors in pearl millet grains: Phytate and goitrogens content variability and molecular characterization of genes involved in their pathways. PloS one (TSI), 13 (6). pp. 1-30. ISSN 1932-6203

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Pearl millet [Pennisetum glaucum (L.) R. Br.] is an important “orphan” cereal and the most widely grown of all the millet species worldwide. It is also the sixth most important cereal in the world after wheat, rice, maize, barley, and sorghum, being largely grown and used in West Africa as well as in India and Pakistan. The present study was carried out in the frame of a program designed to increase benefits and reduce potential health problems deriving from the consumption of pearl millet. The specific goal was to provide a database of information on the variability existing in pearl millet germplasm as to the amounts of phytate, the most relevant antinutrient compound, and the goitrogenic compounds C-glycosylflavones (C-GFs) accumulated in the grain.Results we obtained clearly show that, as indicated by the range in values, a substantial variability subsists across the investigated pearl millet inbred lines as regards the grain level of phytic acid phosphate, while the amount of C-GFs shows a very high variation. Suitable potential parents to be used in breeding programs can be therefore chosen from the surveyed material in order to create new germplasm with increased nutritional quality and food safety. Moreover, we report novel molecular data showing which genes are more relevant for phytic acid biosynthesis in the seeds as well as a preliminary analysis of a pearl millet orthologous gene for C-GFs biosynthesis. These results open the way to dissect the genetic determinants controlling key seed nutritional phenotypes and to the characterization of their impact on grain nutritional value in pearl millet.

Item Type: Article
Divisions: Research Program : West & Central Africa
CRP: CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC)
Uncontrolled Keywords: Phytic acid determination, pearl millet grains, glycosylflavones, phytic acid biosynthesis, Grain phytic acid, grain nutritional value
Subjects: Others > Molecular Biology
Others > Plant Breeding
Mandate crops > Millets > Pearl Millet
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 15 Jun 2018 04:44
Last Modified: 30 Oct 2018 10:28
Official URL:
Acknowledgement: We wish to thank Dr. Yves Vigouroux, UMR DIADE, Montpellier, France, for accessing pearl millet genome sequence before its release, Prof. Fausto Feletti, Dipartimento di Medicina Interna e Terapia, Università di Pavia, Italy, for the precious advices he gave us about optimization of the HPLC method for the detection of CGFs and Dr. Laurent Laplaze, UMR DIADE, Montpellier, France, for critical reading of the manuscript. Funding: This work was funded by the NewPearl grant funded in the frame of the CERES joint initiative by Agropolis Fondation (NÊ AF 1301-015) and by Fondazione Cariplo (NÊ FC 2013-0891 to FS and EN).
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