eprintid: 9931
rev_number: 12
eprint_status: archive
userid: 1305
dir: disk0/00/00/99/31
datestamp: 2017-03-31 09:31:53
lastmod: 2017-04-18 05:57:33
status_changed: 2017-03-31 09:31:53
type: article
metadata_visibility: show
creators_name: Hima Kumari, P
creators_name: Anil Kumar, S
creators_name: Sivan, P
creators_name: Katam, R
creators_name: Suravajhala, P
creators_name: Rao, K S
creators_name: Varshney, R K
creators_name: Kavi Kishor, P B
icrisatcreators_name: Varshney, R K
affiliation: Department of Genetics, Osmania University (Hyderabad)
affiliation: Department of Biosciences, Sardar Patel University (Anand)
affiliation: Department of Biological Sciences, College of Science and Technology, Florida A&M University (Tallahassee)
affiliation: Bioclues Organization (Hyderabad)
affiliation: ICRISAT (Patancheru)
country: India
title: Overexpression of a Plasma Membrane Bound Na+/H+ Antiporter-Like Protein (SbNHXLP) Confers Salt Tolerance and Improves Fruit Yield in Tomato by Maintaining Ion Homeostasis
ispublished: pub
subjects: s2.13
divisions: CRPS3
crps: crp1.5
full_text_status: public
keywords: SbNHXLP, tomato, salt stress, co-immunoprecipitation, CHX2
note: This study was supported by grants from the Department of Science and Technology (DST No: SR/SO/PS-55/07), New Delhi. PHK is thankful to the DST and UGC for providing fellowship. PBK is grateful to the CSIR, New Delhi, for providing Emeritus-fellowship.We thank Dr. Rajagopal, University of Hyderabad, Hyderabad for permitting us to use Handy plant efficiency analyzer.
abstract: A Na+/H+ antiporter-like protein (NHXLP) was isolated from Sorghum bicolor L. (SbNHXLP) and validated by overexpressing in tomato for salt tolerance. Homozygous T2 transgenic lines when evaluated for salt tolerance, accumulated low Na+ and displayed enhanced salt tolerance compared to wild-type plants (WT). This is consistent with the amiloride binding assay of the protein. Transgenics exhibited higher accumulation of proline, K+, Ca2+, improved cambial conductivity, higher PSII, and antioxidative enzyme activities than WT. Fluorescence imaging results revealed lower Na+ and higher Ca2+ levels in transgenic roots. Co-immunoprecipitation experiments demonstrate that SbNHXLP interacts with a Solanum lycopersicum cation proton antiporter protein2 (SlCHX2). qRT-PCR results showed upregulation of SbNHXLP and SlCHX2 upon treatment with 200 mM NaCl and 100 mM potassium nitrate. SlCHX2 is known to be involved in K+ acquisition, and the interaction between these two proteins might help to accumulate more K+ ions, and thus maintain ion homeostasis. These results strongly suggest that plasma membrane bound SbNHXLP involves in Na+ exclusion, maintains ion homeostasis in transgenics in comparison with WT and alleviates NaCl stress.
date: 2017-01
date_type: published
publication: Frontiers in Plant Science
volume: 7
number: 2027
publisher: Frontiers Media
pagerange: 1-15
id_number: 10.3389/fpls.2016.02027
refereed: TRUE
issn: 1664-462X
official_url: http://dx.doi.org/10.3389/fpls.2016.02027
related_url_url: https://scholar.google.co.in/scholar?q=Overexpression+of+a+Plasma+Membrane+Bound+Na%2B%2FH%2B+Antiporter-Like+Protein+%28SbNHXLP%29+Confers+Salt+Tolerance+and+Improves+Fruit+Yield+in+Tomato+by+Maintaining+Ion+Homeostasis+&btnG=&hl=en&as_sdt=0%2C5
related_url_type: pub
funders: Department of Science and Technology
citation:   Hima Kumari, P and Anil Kumar, S and Sivan, P and Katam, R and Suravajhala, P and Rao, K S and Varshney, R K and Kavi Kishor, P B  (2017) Overexpression of a Plasma Membrane Bound Na+/H+ Antiporter-Like Protein (SbNHXLP) Confers Salt Tolerance and Improves Fruit Yield in Tomato by Maintaining Ion Homeostasis.  Frontiers in Plant Science, 7 (2027).  pp. 1-15.  ISSN 1664-462X     
document_url: http://oar.icrisat.org/9931/1/fpls-07-02027.pdf