eprintid: 9903
rev_number: 13
eprint_status: archive
userid: 1305
dir: disk0/00/00/99/03
datestamp: 2017-03-10 11:04:48
lastmod: 2017-03-10 11:18:35
status_changed: 2017-03-10 11:04:48
type: article
metadata_visibility: show
creators_name: Mbuvi, D A
creators_name: Masiga, C W
creators_name: Kuria, E
creators_name: Masanga, J
creators_name: Wamalwa, M
creators_name: Mohamed, A
creators_name: Odeny, D A
creators_name: Hamza, N
creators_name: Timko, M P
creators_name: Runo, S
icrisatcreators_name: Odeny, D A
affiliation: Department of Biochemistry and Biotechnology, Kenyatta University (Nairobi)
affiliation: Tropical Institute of Development Innovations (Kampala)
affiliation: Sudan Academy of Sciences (Khartoum)
affiliation: Agricultural Research Cooperation (Wad-Medani)
affiliation: ICRISAT (Nairobi)
affiliation: Commission for Biotechnology and Genetic Engineering, National Centre for Research (Khartoum)
affiliation: Department of Biology, University of Virginia (Charlottesville)
country: Kenya
country: Uganda
country: Sudan
country: USA
title: Novel Sources of Witchweed (Striga) Resistance from Wild Sorghum Accessions
ispublished: pub
subjects: s1.4
divisions: CRPS5
crps: crp1.4
full_text_status: public
keywords: Striga resistance; witchweed; sorghum; wild sorghum relatives; Sub-Saharan Africa Nomenclature: Striga hermonthica benth.; witchweed; sorghum bicolor
note: The National Academies of Science (NAS) supported this research project under the Partnerships Enhanced Engagement in Research (PEER) program (contract number NAS Sub-Grant Award Letter Agreement Number PGA-2000003439). The Sub-Grant Agreement was funded under Prime Agreement Number AID-OAA-A-11-00012, which was entered into by and between the NAS and the United States Agency for International Development (USAID). MPT was supported by grants from the National Science Foundation (DBI-0701748 and IBN-0322420). We further acknowledge the fieldwork support we received from the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT).
abstract: Sorghum is a major food staple in sub-Saharan Africa (SSA), but its production is constrained by the parasitic plant Striga that attaches to the roots of many cereals crops and causes severe stunting and loss of yield. Away from cultivated farmland, wild sorghum accessions grow as weedy plants and have shown remarkable immunity to Striga. We sought to determine the extent of the resistance to Striga in wild sorghum plants. Our screening strategy involved controlled laboratory assays of rhizotrons, where we artificially infected sorghum with Striga, as well as field experiments at three sites, where we grew sorghum with a natural Striga infestation. We tested the resistance response of seven accessions of wild sorghum of the aethiopicum, drummondii, and arundinaceum races against N13, which is a cultivated Striga resistant landrace. The susceptible control was farmer-preferred variety, Ochuti. From the laboratory experiments, we found three wild sorghum accessions (WSA-1, WSE-1, and WSA-2) that had significantly higher resistance than N13. These accessions had the lowest Striga biomass and the fewest and smallest Striga attached to them. Further microscopic and histological analysis of attached Striga haustorium showed that wild sorghum accessions hindered the ingression of Striga haustorium into the host endodermis. In one of the resistant accessions (WSE-1), host and parasite interaction led to the accumulation of large amounts of secondary metabolites that formed a dark coloration at the interphase. Field experiments confirmed the laboratory screening experiments in that these same accessions were found to have resistance against Striga. In the field, wild sorghum had low Area under the Striga Number Progressive curve (AUSNPC), which measures emergence of Striga from a host over time. We concluded that wild sorghum accessions are an important reservoir for Striga resistance that could be used to expand the genetic basis of cultivated sorghum for resistance to the parasite.
date: 2017-02
date_type: published
publication: Frontiers in Plant Science
volume: 8
number: 116
publisher: Frontiers Media
pagerange: 1-15
id_number: 10.3389/fpls.2017.00116
refereed: TRUE
issn: 1664-462X
official_url: http://dx.doi.org/10.3389/fpls.2017.00116
related_url_url: https://scholar.google.co.in/scholar?hl=en&q=Novel+Sources+of+Witchweed+%28Striga%29+Resistance+from+Wild+Sorghum+Accessions&btnG=
related_url_type: pub
citation:   Mbuvi, D A and Masiga, C W and Kuria, E and Masanga, J and Wamalwa, M and Mohamed, A and Odeny, D A and Hamza, N and Timko, M P and Runo, S  (2017) Novel Sources of Witchweed (Striga) Resistance from Wild Sorghum Accessions.  Frontiers in Plant Science, 8 (116).  pp. 1-15.  ISSN 1664-462X     
document_url: http://oar.icrisat.org/9903/1/fpls-08-00116.pdf