TY - JOUR AV - restricted A1 - Yoneyama, T A1 - Nambiar, P T C A1 - Lee, K K A1 - Srinivasa Rao, B A1 - Williams, J H TI - Nitrogen accumulation in three legumes and two cereals with emphasis on estimation of N2 fixation in the legumes by the natural 15N-abundance technique UR - http://dx.doi.org/10.1007/BF00335857 JF - Biology and Fertility of Soils SN - 0178-2762 PB - Springer-Verlag N1 - ICRISAT Journal Article No. 876 N2 - N accumulation and natural 15N abundance in three legumes (groundnuts, cowpeas, and soybeans) and in two cereals (sorghum and maize) were investigated over two seasons in Alfisols with and without N fertilization. Using the N uptake and natural 15N abundance of non-nodulating plants as the indication of N derived from soil and fertilizer, the per cent N derived from atmospheric N2 was calculated for nodulated plants. In the first experiment, the groundnut genotype contained 85% atmosphere-derived N, but the percentage decreased with N application. Estimates of atmosphere-derived N by the N-difference and 15N-abundance techniques gave identical results. The percentages of atmosphere-derived N estimated by the two methods at different stages of groundnut growth were also similar. In the second experiment, atmosphere-derived N was estimated in plants grown with 0?200 kg ha-1 applied N. The estimated atmosphere-derived N ranged from 42% to 61% for groundnuts from 33% to 77% for cowpeas, and from 24% to 48% for soybeans, depending on the amount of N applied. Inoculation with a Brady rhizobium strain increased the percentage of atmosphere derived N in soybean plants grown without any fertilizer N. The natural 15N abundance of sorghum and maize was very close to that of the non-nodulating groundnut, suggesting that these cereals can be used as reference plants in the estimation of atmosphere-derived N by the natural 15N-abundance method. KW - N2 fixation KW - Natural 15N abundance KW - N-difference method KW - Atmosphere-derived N KW - Non-nodulating genotype KW - Cereals KW - Legumes Y1 - 1990/02// SP - 25 ID - icrisat9704 EP - 30 VL - 09 IS - 01 ER -