eprintid: 9697
rev_number: 10
eprint_status: archive
userid: 1305
dir: disk0/00/00/96/97
datestamp: 2016-09-26 06:19:01
lastmod: 2016-11-09 10:45:15
status_changed: 2016-09-26 06:19:01
type: article
metadata_visibility: show
creators_name: Ramalingam, A
creators_name: Kudapa, H
creators_name: Pazhamala, L T
creators_name: Garg, V
creators_name: Varshney, R K
icrisatcreators_name: Ramalingam, A
icrisatcreators_name: Kudapa, H
icrisatcreators_name: Pazhamala, L T
icrisatcreators_name: Garg, V
icrisatcreators_name: Varshney, R K
affiliation: ICRISAT (Patancheru)
affiliation: School of Plant Biology and Institute of Agriculture, The University of Western Australia (Crawley)
country: India
country: Australia
title: Gene Expression and Yeast Two-Hybrid Studies of 1R-MYB Transcription Factor Mediating Drought Stress Response in Chickpea (Cicer arietinum L.)
ispublished: pub
subjects: s1.1
subjects: s2.13
divisions: CRPS3
crps: crp1.5
full_text_status: public
keywords: Protein-protein interactions, Chickpea, Transcription factor, Drought, Stress tolerance, Signaling pathways
note: AR is grateful for the Australia-India Early Career Fellowship by the Australian Academy of Science. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium.
abstract: Drought stress has been one of the serious constraints affecting chickpea productivity to a great extent. Genomics-assisted breeding has a potential to accelerate breeding precisely and efficiently. In order to do so, understanding the molecular mechanisms for drought tolerance and identification of candidate genes are crucial. Transcription factors (TFs) have important roles in the regulation of plant stress related genes. In this context, quantitative real time-PCR (qRT-PCR) was used to study the differential gene expression of selected TFs, identified from large-scale expressed sequence tags (ESTs) analysis, in contrasting drought responsive genotypes. Root tissues of ICC 4958 (tolerant), ICC 1882 (sensitive), JG 11 (elite), and JG 11+ (introgression line) were used for the study. Subsequently, a candidate single repeat MYB (1R-MYB) transcript that was remarkably induced in the drought tolerant genotypes under drought stress was cloned (coding sequence region for the 1R-MYB protein) and subjected to yeast two-hybrid (Y2H) analysis. The screening of a root cDNA library with Y2H using the 1R-MYB bait protein, identified three CDS encoding peptides namely, galactinol-sucrose galactosyltransferase 2, CBL (Calcineurin B-like)-interacting serine/threonine-protein kinase 25, and ABA responsive 17-like, which were confirmed by co-transformation in yeast. These findings provide preliminary insights into the ability of this 1R-MYB transcription factor to co-regulate drought tolerance mechanism in chickpea.
date: 2015-12-24
date_type: published
publication: Frontiers in Plant Science
volume: 6
number: 1117
publisher: Frontiers Media
pagerange: 01-10
id_number: 10.3389/fpls.2015.01117
refereed: TRUE
issn: 1664-462X
official_url: http://dx.doi.org/10.3389/fpls.2015.01117
related_url_url: https://scholar.google.co.in/scholar?q=Gene+expression+and+Yeast+two-hybrid+studies+of+a+1RMYB+transcription+factor+mediating+drought+stress+response+in+root+tissues+of+chickpea+%28Cicer+arietinum+L.%29&btnG=&hl=en&as_sdt=0%2C5
related_url_type: pub
citation:   Ramalingam, A and Kudapa, H and Pazhamala, L T and Garg, V and Varshney, R K  (2015) Gene Expression and Yeast Two-Hybrid Studies of 1R-MYB Transcription Factor Mediating Drought Stress Response in Chickpea (Cicer arietinum L.).  Frontiers in Plant Science, 6 (1117).  01-10.  ISSN 1664-462X     
document_url: http://oar.icrisat.org/9697/1/fpls-06-01117.pdf