eprintid: 9567
rev_number: 8
eprint_status: archive
userid: 1305
dir: disk0/00/00/95/67
datestamp: 2016-06-24 06:52:31
lastmod: 2016-06-24 06:52:31
status_changed: 2016-06-24 06:52:31
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
creators_name: Reddy, S V
creators_name: Kiran Mayi, D
creators_name: Uma Reddy, M
creators_name: Thirumala-Devi, K
creators_name: Reddy, D V R
icrisatcreators_name: Reddy, S V
affiliation: ICRISAT (Patancheru)
affiliation: Department of Foods and Nutrition, College of Home Science, Acharya N. G. Ranga Agricultural University (Hyderabad)
country: India
title: Aflatoxins B1 in different grades of chillies (Capsicum annum L.) in India as determined by indirect competitive-ELISA
ispublished: pub
subjects: s2
full_text_status: restricted
keywords: Chillies Spices, Aflatoxin, B1, Indirect Competitive-ELISA, Chillies
abstract: Samples of the three grades of chilli pod (grades 1 to 3) were collected during surveys in 1998 and 1999 from the principal market yards and cold storage facilities of the major chilli-growing areas of Andhra Pradesh (AP), India. Chilli powders were collected from different supermarkets in Hyderabad, AP. They were analysed for aflatoxin B1 (AFB1) content by an indirect competitive ELISA. To avoid the influence of interfering substances present in chilli extracts, it was necessary to prepare the aflatoxin standards in methanol extracts of chillies free from aflatoxins. For nine representative samples there was good agreement between ELISA and HPLC estimations of AFB1 and the results suggested that the ELISA procedure adopted was dependable. Of the 182 chilli samples tested, 59% of the samples were contaminated with AFB1 and 18% contained the toxin at non-permissible levels. The highest AFB1 concentration of 969 µg/kg was found in one sample representing grade 3. Overall the maximum percentage of chilli pods showing AFB1 levels higher than 30 μg/kg (non-permissible levels) was in grade 3. Chilli pods stored in refrigerated rooms showed the lowest proportion of samples containing aflatoxin. Nearly 9% of the chilli powders sold in supermarkets contained non-permissible aflatoxin levels. This report highlights the importance of using grade 1 chilli pods to minimize aflatoxin contamination.
date: 2001
date_type: published
publication: Food Additives and Contaminants
volume: 18
number: 06
publisher: Taylor & Francis
pagerange: 553-558
refereed: TRUE
issn: 0265-203X
official_url: http://dx.doi.org/10.1080/02652030119491
citation:   Reddy, S V and Kiran Mayi, D and Uma Reddy, M and Thirumala-Devi, K and Reddy, D V R  (2001) Aflatoxins B1 in different grades of chillies (Capsicum annum L.) in India as determined by indirect competitive-ELISA.  Food Additives and Contaminants, 18 (06).  pp. 553-558.  ISSN 0265-203X     
document_url: http://oar.icrisat.org/9567/1/Food%20Additives%20and%20Contaminants.pdf