http://oar.icrisat.org/id/eprint/9436 2016-10-21T09:35:55Z info:eu-repo/semantics/descriptiveMetadata http://oar.icrisat.org/9436/ Genetic dissection of seed-iron and zinc concentrations in chickpea Upadhyaya, H D Bajaj, D Das, S Kumar, V Gowda, C L L Sharma, S Tyagi, A K Parida, S K Chickpea Food Legumes Genetics and Genomics The SNP-based high-resolution QTL mapping mapped eight major genomic regions harbouring robust QTLs governing seed-Fe and Zn concentrations (39.4% combined phenotypic variation explained/PVE) on six chromosomes of an intra-specific high-density genetic linkage map (1.56 cM map-density). 24620 SNPs discovered from genome-wide GBS (genotyping-by-sequencing) and 13 known cloned Fe and Zn contents-related chickpea gene-orthologs were genotyped in a structured population of 92 sequenced desi and kabuli accessions. The large-scale 16591 SNP genotyping- and phenotyping-based GWAS (genome-wide association study) identified 16 genomic loci/genes associated (29% combined PVE) with seed-Fe and Zn concentrations. Of these, 11 trait-associated SNPs in the genes linked tightly with eight QTLs were validated by QTL mapping. The seed-specific expression, including pronounced differential-regulation of 16 trait-associated genes particularly in accessions/mapping individuals with contrasting level of seed-Fe and Zn contents was apparent. Collectively, the aforementioned rapid integrated genomic strategy led to delineate novel functional non-synonymous and regulatory SNP allelic-variants from 16 known/candidate genes, including three strong trait-associated genes (encoding late embryogenesis abundant and yellow stripe-like 1 protein, and vacuolar protein sorting-associated protein) and eight major QTLs regulating seed-Fe and Zn concentrations in chickpea. These essential inputs thus have potential to be deployed in marker-assisted genetic enhancement for developing nutritionally-rich iron/zinc-biofortified chickpea cultivars. Nature Publishing Group 2016-04-11 Article PeerReviewed application/pdf en http://oar.icrisat.org/9436/1/srep24050.pdf Upadhyaya, H D and Bajaj, D and Das, S and Kumar, V and Gowda, C L L and Sharma, S and Tyagi, A K and Parida, S K (2016) Genetic dissection of seed-iron and zinc concentrations in chickpea. Scientific Reports, 06 (24050). 01-12. ISSN 2045-2322 http://dx.doi.org/10.1038/srep24050 10.1038/srep24050 info:eu-repo/semantics/objectFile info:eu-repo/semantics/humanStartPage