"9436","12","archive","1305",,,"disk0/00/00/94/36","2016-04-18 08:24:51","2016-10-21 09:35:55","2016-04-18 08:24:51","article",,,"show",,,,"","","","","","","","","","",,,,"Upadhyaya","H D","","","","","","","Upadhyaya","H D","","","","",,,,,"","",,,,,"","","ICRISAT (Patancheru)","India","Genetic dissection of seed-iron and zinc concentrations in chickpea","pub","s1.1","D3","crp1.5","public",,,"Genetic markers, Genome-wide association studies, Genetics, QTL mapping, Chickpea",,"The authors gratefully acknowledge the financial support for this study provided by a research grant from the
Department of Biotechnology (DBT), Government of India (102/IFD/SAN/2161/2013-14). Shouvik Das
acknowledges the DBT for Senior Research Fellowship award","The SNP-based high-resolution QTL mapping mapped eight major genomic regions harbouring robust QTLs governing seed-Fe and Zn concentrations (39.4% combined phenotypic variation explained/PVE) on six chromosomes of an intra-specific high-density genetic linkage map (1.56 cM map-density). 24620 SNPs discovered from genome-wide GBS (genotyping-by-sequencing) and 13 known cloned Fe and Zn contents-related chickpea gene-orthologs were genotyped in a structured population of 92 sequenced desi and kabuli accessions. The large-scale 16591 SNP genotyping- and phenotyping-based GWAS (genome-wide association study) identified 16 genomic loci/genes associated (29% combined PVE) with seed-Fe and Zn concentrations. Of these, 11 trait-associated SNPs in the genes linked tightly with eight QTLs were validated by QTL mapping. The seed-specific expression, including pronounced differential-regulation of 16 trait-associated genes particularly in accessions/mapping individuals with contrasting level of seed-Fe and Zn contents was apparent. Collectively, the aforementioned rapid integrated genomic strategy led to delineate novel functional non-synonymous and regulatory SNP allelic-variants from 16 known/candidate genes, including three strong trait-associated genes (encoding late embryogenesis abundant and yellow stripe-like 1 protein, and vacuolar protein sorting-associated protein) and eight major QTLs regulating seed-Fe and Zn concentrations in chickpea. These essential inputs thus have potential to be deployed in marker-assisted genetic enhancement for developing nutritionally-rich iron/zinc-biofortified chickpea cultivars.","2016-04-11","published",,"Scientific Reports","06","24050","Nature Publishing Group",,"01-12",,,,,,"10.1038/srep24050",,,,,"TRUE",,"2045-2322",,,,,,"","http://dx.doi.org/10.1038/srep24050","https://scholar.google.co.in/scholar?hl=en&q=Genetic+dissection+of+seed-iron+and+zinc+concentrations+in+chickpea&btnG=","pub",,"Department of Biotechnology (DBT), Government of India","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
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