eprintid: 9122
rev_number: 8
eprint_status: archive
userid: 17
dir: disk0/00/00/91/22
datestamp: 2015-11-09 09:15:58
lastmod: 2015-11-09 09:15:58
status_changed: 2015-11-09 09:15:58
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
creators_name: Kaushal, M
creators_name: Kaushal, R
icrisatcreators_name: Kaushal, M
affiliation: ICRISAT (Patancheru)
affiliation: Dr Y S Parmar University of Horticulture and Forestry (Nauni)
country: India
title: Acetylene reductase activity and molecular characterization of plant growth promoting rhizobacteria to know efficacy in integrated nutrient management system
ispublished: pub
subjects: s2.17
subjects: s2.4
divisions: D5
crps: crp1.1
full_text_status: restricted
keywords: Acetylene reduction assay (ARA), Bacillus spp., Biocontrol, Cauliflower, INM, PGPR, 16S rRNA
abstract: Isolates from rhizosphere were screened and characterized for their plant growth promotion and biocontrol properties as per standard methods. Five isolates (MK2, MK4, MK5, MK7 & MK9) showed the maximum plant growth promoting attributes like P-solubilization, N-fixation etc. Acetylene reduction assay (ARA) method was used to study N2 fixing ability. The bacterial isolate MK5 fixed the maximum nitrogen, i.e., 437.26 ηmole C2H4 h-1 mg-1 protein (109 mg of N2 fixed/ha/d). The isolates MK5 and MK7 showed the maximum homology with Bacillus pumilus and B. subtilis, respectively by 16S rRNA analysis. The bacterial isolate MK5 in conjunction with 75% level of recommended dose of N and P fertilizers gave increase yield of cauliflower crop by 27.29% over recommended doses of NPK with a saving of 25% N and 25% P fertilizers.
date: 2015
date_type: published
publication: Indian Journal of Biotechnology
volume: 14
number: 2
publisher: NISCAIR
pagerange: 221-227
refereed: TRUE
issn: 0975-0967
related_url_url: http://scholar.google.co.in/scholar?as_q=Acetylene+reductase+activity+and+molecular+characterization+of+plant+growth+promoting+rhizobacteria+to+know+efficacy+in+integrated+nutrient+management+system&as_epq=&as_oq=&as_eq=&as_occt=title&as_sauthors=&as_publ
related_url_type: pub
citation:   Kaushal, M and Kaushal, R  (2015) Acetylene reductase activity and molecular characterization of plant growth promoting rhizobacteria to know efficacy in integrated nutrient management system.  Indian Journal of Biotechnology, 14 (2).  pp. 221-227.  ISSN 0975-0967     
document_url: http://oar.icrisat.org/9122/1/IJB_14_221-227_2015.pdf