TY - JOUR AV - restricted UR - http://oar.icrisat.org/9122/ IS - 2 PB - NISCAIR TI - Acetylene reductase activity and molecular characterization of plant growth promoting rhizobacteria to know efficacy in integrated nutrient management system A1 - Kaushal, M A1 - Kaushal, R SN - 0975-0967 VL - 14 N2 - Isolates from rhizosphere were screened and characterized for their plant growth promotion and biocontrol properties as per standard methods. Five isolates (MK2, MK4, MK5, MK7 & MK9) showed the maximum plant growth promoting attributes like P-solubilization, N-fixation etc. Acetylene reduction assay (ARA) method was used to study N2 fixing ability. The bacterial isolate MK5 fixed the maximum nitrogen, i.e., 437.26 ?mole C2H4 h-1 mg-1 protein (109 mg of N2 fixed/ha/d). The isolates MK5 and MK7 showed the maximum homology with Bacillus pumilus and B. subtilis, respectively by 16S rRNA analysis. The bacterial isolate MK5 in conjunction with 75% level of recommended dose of N and P fertilizers gave increase yield of cauliflower crop by 27.29% over recommended doses of NPK with a saving of 25% N and 25% P fertilizers. SP - 221 Y1 - 2015/// EP - 227 ID - icrisat9122 KW - Acetylene reduction assay (ARA) KW - Bacillus spp. KW - Biocontrol KW - Cauliflower KW - INM KW - PGPR KW - 16S rRNA JF - Indian Journal of Biotechnology ER -