%0 Journal Article
%@ 0975-0967
%A Kaushal, M
%A Kaushal, R
%D 2015
%F icrisat:9122
%I NISCAIR
%J Indian Journal of Biotechnology
%K Acetylene reduction assay (ARA), Bacillus spp., Biocontrol, Cauliflower, INM, PGPR, 16S rRNA
%N 2
%P 221-227
%T Acetylene reductase activity and molecular characterization of plant growth promoting rhizobacteria to know efficacy in integrated nutrient management system
%U http://oar.icrisat.org/9122/
%V 14
%X Isolates from rhizosphere were screened and characterized for their plant growth promotion and biocontrol properties as per standard methods. Five isolates (MK2, MK4, MK5, MK7 & MK9) showed the maximum plant growth promoting attributes like P-solubilization, N-fixation etc. Acetylene reduction assay (ARA) method was used to study N2 fixing ability. The bacterial isolate MK5 fixed the maximum nitrogen, i.e., 437.26 ηmole C2H4 h-1 mg-1 protein (109 mg of N2 fixed/ha/d). The isolates MK5 and MK7 showed the maximum homology with Bacillus pumilus and B. subtilis, respectively by 16S rRNA analysis. The bacterial isolate MK5 in conjunction with 75% level of recommended dose of N and P fertilizers gave increase yield of cauliflower crop by 27.29% over recommended doses of NPK with a saving of 25% N and 25% P fertilizers.